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Anonymous ID: 6c1544 Feb. 11, 2021, 6:45 a.m. No.12891548   🗄️.is 🔗kun   >>1559 >>1562

>>12891521

>>12891512

 

Since no quantified virus isolates of the 2019-nCoV are currently available, assays designed for detection of the 2019-nCoV RNA were tested with characterized stocks of in vitro transcribed full length RNA (N gene; GenBank accession: MN908947.2) of known titer (RNA copies/μL) spiked into a diluent consisting of a suspension of human A549 cells and viral transport medium (VTM) to mimic clinical specimen”.

 

https://www.ncbi.nlm.nih.gov/nuccore/MN908947.2

Wuhan seafood market pneumonia virus isolate Wuhan-Hu-1, complete genome

Anonymous ID: 6c1544 Feb. 11, 2021, 6:48 a.m. No.12891562   🗄️.is 🔗kun   >>1580 >>1718 >>1824 >>1975 >>2087 >>2105

>>12891548

>human A549 cells

https://en.wikipedia.org/wiki/A549_cell

 

A549 cells are adenocarcinomic human alveolar basal epithelial cells, and constitute a cell line that was first developed in 1972 by D. J. Giard, et al. through the removal and culturing of cancerous lung tissue in the explanted tumor of a 58-year-old caucasian male. The cells are used as models for the study of lung cancer and the development of drug therapies against it.