Anonymous ID: f34ed0 Feb. 15, 2021, 1:01 p.m. No.12935865   🗄️.is 🔗kun   >>5879 >>5898

ok guise

remember all the minks that were killed due to covid

well

I found this on a webpage for checking DNA (not cheap) (and what a racket this DNA shit creates all kinds of $$$ grift)

for your pet cat

to see what breed your cat may be

 

then it occurred to me eventually the fake news needs new scary things to hype and prop up covid scare

how long until the all the fake news

'takes this information regarding

feline corona virus

and panics the world over cats

OR

adds cats corona to total cases

(joking but not joking)

 

it is estimated 58,385,725 cats in the USA

so, i bet the scammers at the cdc and fake news outlets

are looking out for ways to get these feline numbers into the dialogue around covid panic

if they do it will cost a lot of cats to be killed by people who already hate cats

and then brainwashed who will fear cats as a new fear and harm everyday cats as well

 

hope i a m wrong

 

https://basepaws.com/pages/science

 

11/24/2020

 

https://www.medrxiv.org/content/medrxiv/early/2020/11/24/2020.11.21.20236216.full.pdf

 

here is just the first page:

 

Use of alternative RNA storage and extraction reagents and development of a

2 hybrid PCR-based method for SARS-CoV-2 detection

 

Julie Yang1

, Elias Salfati

1

, Damian Kao*

1

, Yuliana Mihaylova*

1

5

6 Basepaws, 1124 W Carson Street, MRL Building, 3rd floor, Torrance, CA 90502

 

Abstract

36 The COVID-19 pandemic has presented multiple healthcare challenges, one of which is

37 adequately meeting the need for large-scale diagnostic testing. The most commonly used

38 assays for detection of SARS-CoV-2, including those recommended by the Center for Disease

39 Control and Prevention (CDC), rely on a consistent set of core reagents. This has put a serious

40 strain on the reagent supply chain, resulting in insufficient testing. '''It has also led to restricted

41 animal testing, even though there are now multiple reports of animals, particularly cats,''' ferrets

42 and minks, contracting the disease. We aimed to address the diagnostic bottleneck by

43 developing a PCR-based SARS-CoV-2 detection assay for cats (and, potentially, other animals)

44 which avoids the use of most common reagents, such as collection kits optimized for RNA

45 stabilization, RNA isolation kits and TaqMan-based RT-PCR reagents. We demonstrated that an

46 inexpensive solid-phase reversible immobilization (SPRI) method can be used for RNA

47 extraction from feline samples collected with DNAGenotek’s ORAcollect RNA OR-100 and

48 PERFORMAgene DNA PG-100 sample collection kits, optimized for RNA or DNA stabilization,

49 respectively. We developed a dual method SARS-CoV-2 detection assay relying on SYBR

50 RT-PCR and Sanger sequencing, using the same set of custom synthesized oligo primers. We

51 validated our test’s specificity with a commercially available SARS-CoV-2 plasmid positive

52 control, as well as two in-house positive control RNA samples. Our assay’s sensitivity was

53 determined to be 10 viral copies per reaction. Our results suggest that a simple

54 SPRI-dependent RNA extraction protocol and certain sample collection kits not specifically

55 optimized for RNA stabilization could potentially be used in cases where reagent shortages are

56 hindering adequate COVID-19 testing. These ‘alternative’ reagents could be used in

57 combination with our COVID-19 testing method, which relies on inexpensive and readily

58 available SYBR RT-PCR and non-fluorescent PCR reagents. Depending on the detection goals

59 and the laboratory setup available, the SYBR RT-PCR method and the Sanger sequencing

60 based method can be used alone or in conjunction, for improved accuracy. '''Although the test is

61 intended for animal use, it is, in theory, possible to use it with human samples,''' especially those

62 with higher viral loads.

 

more here: https://www.medrxiv.org/content/medrxiv/early/2020/11/24/2020.11.21.20236216.full.pdf

Anonymous ID: f34ed0 Feb. 15, 2021, 1:02 p.m. No.12935879   🗄️.is 🔗kun   >>5898

>>12935865

>The COVID-19 pandemic has presented multiple healthcare challenges, one of which is

 

>37 adequately meeting the need for large-scale diagnostic testing. The most commonly used

 

>38 assays for detection of SARS-CoV-2, including those recommended by the Center for Disease

 

>39 Control and Prevention (CDC), rely on a consistent set of core reagents. This has put a serious

 

>40 strain on the reagent supply chain, resulting in insufficient testing. '''It has also led to restricted

 

>41 animal testing, even though there are now multiple reports of animals, particularly cats,''' ferrets

 

>42 and minks, contracting the disease. We aimed to address the diagnostic bottleneck by

 

>43 developing a PCR-based SARS-CoV-2 detection assay for cats (and, potentially, other animals)

Anonymous ID: f34ed0 Feb. 15, 2021, 1:05 p.m. No.12935898   🗄️.is 🔗kun

>>12935865

>>12935879

 

potentially OTHER ANIMALS

 

see the grift coming down the pike

using DNA and covid

money and scare angles everywhere on the horizon

for the grifters

 

> developing a PCR-based SARS-CoV-2 detection assay for cats (and, potentially, other animals)