Anonymous ID: 86c1ae May 3, 2021, 10:06 a.m. No.13571745   ๐Ÿ—„๏ธ.is ๐Ÿ”—kun   >>1843 >>1915 >>1935 >>2083 >>2101 >>2251 >>2375 >>2406

Harvard scientists create gene-editing tool that could rival CRISPR

 

Researchers from the Harvard's Wyss Institute for Biologically Inspired Engineering have created a new gene-editing tool that can enable scientists to perform millions of genetic experiments simultaneously. They're calling it the Retron Library Recombineering (RLR) technique, and it uses segments of bacterial DNA called retrons that can produce fragments of single-stranded DNA.

 

When it comes to gene editing, CRISPR-Cas9 is probably the most well-known technique these days. It's been making waves in the science world in the past few years, giving researchers the tool they need to be able to easily alter DNA sequences. It's more accurate than previously used techniques, and it has a wide variety of potential applications, including life-saving treatments for various illnesses.

 

However, the tool has some major limitations. It could be difficult to deliver CRISPR-Cas9 materials in large numbers, which remains a problem for studies and experiments, for one. Also, the way the technique works can be toxic to cells, because the Cas9 enzyme โ€” the molecular "scissors" in charge of cutting strands of DNA โ€” often cuts non-target sites as well.

 

CRISPR-Cas9 physically cuts DNA to incorporate the mutant sequence into its genome during the repair process. Meanwhile, retrons can introduce the mutant DNA strand into a replicating cell, so that the strand can become incorporated into the daughter cells' DNA. Further, retrons' sequences can serve as "barcodes" or "name tags," allowing scientists to track individuals in a pool of bacteria. That means they can be used for genome editing without damaging the native DNA, and they can be used to perform multiple experiments in one big mixture.

 

The Wyss Institute scientists tested RLR on E. coli bacteria and found that 90 percent of the population incorporated the retron sequence after they made a few tweaks. They were also able to prove how useful it can be in massive genetic experiments. During their tests, they were able to find antibiotic resistance mutations in E. coli by sequencing the retrons' barcodes instead of sequencing individual mutants, making the process a lot faster.

 

There's still work to be done before RLR can be widely used, including improving and standardizing its editing rate. The team believes, however, that it can "lead to new, exciting and unexpected innovations."

 

https://news.yahoo.com/harvard-gene-editing-tool-rlr-214700187.html

Anonymous ID: 86c1ae May 3, 2021, 10:18 a.m. No.13571800   ๐Ÿ—„๏ธ.is ๐Ÿ”—kun   >>1815 >>1832 >>1887

>>13571793

1-800-273-8255

Q 2300

 

R

RED OCTOBER

D

Justice K confirmation

DECLAS

Goodbye, Mr. Rosenstein [payment in full]

https://www.cbsnews.com/news/jeff-flake-brett-kavanaugh-fbi-probe-today-elevator-protest-chris-coons-2018-09-28/

"Flake's decision came after he spoke to senators of both parties and withstood pressure from some Republicans not to take such a step. As he considered his course of action, Flake also had a conversation with Deputy Attorney General [Rod Rosenstein], who is in charge of special counsel Robert Mueller's investigation."

Define 'Treason'.

The crime of betraying one's country, especially by attempting to kill the sovereign or overthrow the government.

Define 'Subversion'.

The act of subverting : the state of being subverted; especially : a systematic attempt to overthrow or undermine a government or political system by persons working secretly from within?

Think line of questions re: Sen. Graham Justice K re: 'Enemy Combatants'.

1-800-273-8255

Operators standing by.

Anonymous ID: 86c1ae May 3, 2021, 10:34 a.m. No.13571870   ๐Ÿ—„๏ธ.is ๐Ÿ”—kun   >>1882

>>13571832

Not that I can see.

Timestamp brings up Q 810

 

How do you break up something this big?

What happens if low/mid/senior (non corrupt) Patriots learn they were sold out?

What happens?

Who is waiting with open arms w/ a plan to reorg under a single entity with direct OS by trusted Patriots?

Who are trusted Patriots who understand intel collection?

 

>How do you break up something this big?

Looking at the tweet, whats BIG?

RISK.

Risk bring up a lot of posts: https://qagg.news/?q=risk

Anonymous ID: 86c1ae May 3, 2021, 10:36 a.m. No.13571885   ๐Ÿ—„๏ธ.is ๐Ÿ”—kun   >>1889

@SteveScalise

 

103 days since Biden issued orders that created the border crisis.

 

He hasn't reversed them.

 

40 days since he tapped Harris to solve the crisis.

 

She hasnโ€™t visited the border.

 

39 days since he promised transparency.

 

The press still doesnโ€™t have full access.

 

Think about that.

 

https://twitter.com/SteveScalise/status/1389267397425733632

Anonymous ID: 86c1ae May 3, 2021, 10:49 a.m. No.13571962   ๐Ÿ—„๏ธ.is ๐Ÿ”—kun   >>1979 >>2081 >>2101 >>2251 >>2375 >>2406

@USNavy

 

Now that's what we call a view!

 

#USSBunkerHill (CG 52) conducts routine operations in

the northern Pacific Ocean. Bunker Hill, part of the

Theodore Roosevelt Carrier Strike Group, is on a

scheduled deployment conducting routine operations

in @USPacificFleet.

 

https://twitter.com/USNavy/status/1389263513487618048