Ooh,
Superparamagnetic nanoparticle delivery of DNA vaccine
sounds tasty !
Abstract
The efficiency of delivery of DNA vaccines
is often relatively low compared to protein
vaccines. The use of superparamagnetic
iron oxide nanoparticles (SPIONs) to deliver
genes via magnetofection shows promise in
improving the efficiency of gene delivery
both in vitro and in vivo. In particular, the
duration for gene transfection especially
for in vitro application can be significantly
reduced by magnetofection compared to
the time required to achieve high gene
transfection with standard protocols.
SPIONs that have been rendered stable in
physiological conditions can be used as both
therapeutic and diagnostic agents due to
their unique magnetic characteristics.
Valuable features of iron oxide nanoparticles
in bioapplications include a tight control over
their size distribution, magnetic properties of
these particles, and the ability to carry
particular biomolecules to specific targets.
The internalization and half-life of the
particles within the body depend upon the
method of synthesis. Numerous synthesis
methods have been used to produce magnetic
nanoparticles for bioapplications with
different sizes and surface charges.
The most common method for synthesizing
nanometer-sized magnetite Fe3O4 particles
in solution is by chemical coprecipitation of
iron salts. The coprecipitation method is an
effective technique for preparing a stable
aqueous dispersions of iron oxide nanoparticles.
We describe the production of Fe3O4-based
SPIONs with high magnetization values
(70 emu/g) under 15 kOe of the applied
magnetic field at room temperature,
with 0.01 emu/g remanence via a coprecipitation
method in the presence of trisodium citrate
as a stabilizer. Naked SPIONs often lack
sufficient stability, hydrophilicity, and the
capacity to be functionalized. In order to
overcome these limitations, polycationic
polymer was anchored on the surface of
freshly prepared SPIONs by a direct
electrostatic attraction between the
negatively charged SPIONs (due to the
presence of carboxylic groups) and the
positively charged polymer.
Polyethylenimine was chosen to modify
the surface of SPIONs to assist the delivery
of plasmid DNA into mammalian cells due
to the polymer's extensive buffering capacity
through the "proton sponge" effect.
>>136548
Now given the presence of these
iron oxide nanoparticles in the arms of
these vaccinated people, should they
now avoid receiving MRI's ?
Now given the presence of these
iron oxide nanoparticles in the arms of
these vaccinated people, should they
now avoid receiving MRI's ?