This is a test…saving a power point to mpeg.
>Not an earthquake, but late last night there was supposedly a train derailment in Jupiter FL
I live here…didn't hear about it…but work at home and haven't really been out and about in a day or so… I'll keep my eyes open.
-clockfag
>https://youtu.be/yOKsU0fESUI
well son of a gun…didn't know….
but beeline hwy is towards the middle of nowhere..adn the news report apparently said homicide is taking over….interesting.
usutally, there si a drunk person that tries toa walk across when there is a pedestrian hit….
it's happened a few times in West Palm, Boynton and other places.
>Psaki Says Biden Submits Resignation
excerpt from:
https://pubs.acs.org/doi/suppl/10.1021/acssynbio.9b00077/suppl_file/sb9b00077_si_001.pdf
The PCR product of dxs was digested with XbaI and SpeI and ligated into pSB1C3 cut with XbaI and SpeI toyield pSBS. The correct insertion orientation was confirmed to ensure both digestion sites XbaI and SpeI wereconserved. The PCR product of dxr was digested with NheI and BglII and ligated into NheI/BglII-digestedpSBS to give pSBSR. The PCR product of idi was digested with BglII and ClaI, and ligated into BglII/ClaIdigested pSBSR to give pSBSRI. The PCR product of menA was digested with ClaI and SpeI, and ligated intoClaI/SpeI-digested pSBSRI to give pSBSRIA.The constructed and sequence-verified gene clusters were cut out of the pSB1C3 backbone using XbaI and SpeIand ligated into the SpeI site in pUS258. The construction of pUS258S was not successful using the aboveworkflow possibly due to the selection pressure against the correct orientation of dxs. Therefore, the dxsfragment was cut out of plasmid pUS258SR using XhoI and NheI, and ligated into XhoI/SpeI-digested pUS258to give pUS258S.pUS258R, pUS258I, pUS258AGenes dxr, idi and menA were amplified from plasmid pSBSRIA with Q5 DNA polymerase using primer pairsYMA38/YMA39, YMA40/YMA41 and YMA42/YMA43 respectively. PCR products of dxr, idi and menA weredigested with SalI and PmlI, and ligated into SalI/PmlI-digested pUS258 to give pUS258R, pUS258I, andpUS258A respectively. pUS258SIApSBSRIA was digested with BglII and NheI to remove the dxr fragment, and the plasmid backbone was bluntedwith T4 DNA polymerase and re-ligated back together to give pSBSIA. The resultant dxs-idi-menA gene clusterwas cut out of the plasmid backbone using XbaI and SpeI and ligated into SpeI-digested pUS258 to yieldpUS258SIA.
a little further down:
Supporting Note S3: Routine methods for recombinant DNA work
Gene Amplification from B. subtilis Genome and Purification
Genomic DNA extraction and purification from Bacillus subtilis was performed following a modified FastPrep
protocol 1
PCR amplification of Bacillus subtilis genes dxs, dxr, idi and menA was performed using Q5
polymerase with primer pairs YMA1/YMA2, YMA3/YMA4, YMA5/YMA6, YMA7/YMA8, respectively. Each
25 µL PCR reaction contains 12.5 µL Q5 High Fidelity 2X Master Mix, 1 µL genomic DNA, 1.25 µL of 10 µM
forward primer and reverse primer each, and sterile MilliQ water. The thermocycling conditions for a routine
Q5 PCR started with denaturation at 98°C for 30 seconds, followed by 30 cycles of denaturation, annealing and
extension (98°C for 10 seconds, 55-72°C for 30 seconds and 72°C for 30 seconds/kb of the expected PCR
product), and followed by final extension at 72°C for 5 minutes before holding at 15°C. The resultant PCR
fragments were purified using the ISOLATE II PCR and Gel Kit (Bioline), and the sizes of PCR products were
confirmed via agarose gel electrophoresis
So, I'm sayin, no idea but mebbe PCR test gold and/demonstration of weaponization….but I pree the Psaki says biden submits resignation….