>DOUGH
bat soup
good morning everyone
thank you for coming today
>https://t.me/CodeMonkeyZ/1822
Theranos bat soup?
Is it wet yet?
>https://www.unc.edu/posts/2021/09/23/3d-printed-vaccine-patch/
https://www.pnas.org/content/118/39/e2102595118
Transdermal vaccination via 3D-printed microneedles induces potent humoral and cellular immunity
In summary, we have designed and fabricated a faceted MN array via CLIP 3D printing and established an approach to co-load multiple vaccine components with high tunability. Our model vaccine demonstrated efficient ID delivery of vaccine components as well as the induction of potent and balanced humoral and cellular immunity. The combination of fast 3D-printing technology with MN vaccine formulation could provide a versatile platform to improve global immunization and healthcare.
>https://www.pnas.org/content/118/39/e2102595118
Significance
Vaccination is essential for combatting emerging epidemics and pandemics. Microneedle patches designed to precisely deliver cargos into the intradermal space, rich in immune cells, provide a noninvasive and self-applicable vaccination approach, eliminating the need for hypodermic needles and trained medical personnel for vaccine administration. Here, we show that advanced 3D printing methods allow for the manufacturing of polymeric microneedles of controlled geometries (difficult to manufacture using traditional methods) designed to enhance vaccine component coating. Using model vaccine components, we demonstrated that 3D-printed microneedle delivery resulted in enhanced cargo retention in the skin, activation of immune cells, and more potent humoral and cellular immune responses as compared with traditional vaccination routes.
Abstract
Vaccination is an essential public health measure for infectious disease prevention. The exposure of the immune system to vaccine formulations with the appropriate kinetics is critical for inducing protective immunity. In this work, faceted microneedle arrays were designed and fabricated utilizing a three-dimensional (3D)-printing technique called continuous liquid interface production (CLIP). The faceted microneedle design resulted in increased surface area as compared with the smooth square pyramidal design, ultimately leading to enhanced surface coating of model vaccine components (ovalbumin and CpG). Utilizing fluorescent tags and live-animal imaging, we evaluated in vivo cargo retention and bioavailability in mice as a function of route of delivery. Compared with subcutaneous bolus injection of the soluble components, microneedle transdermal delivery not only resulted in enhanced cargo retention in the skin but also improved immune cell activation in the draining lymph nodes. Furthermore, the microneedle vaccine induced a potent humoral immune response, with higher total IgG (Immunoglobulin G) and a more balanced IgG1/IgG2a repertoire and achieved dose sparing. Furthermore, it elicited T cell responses as characterized by functional cytotoxic CD8+ T cells and CD4+ T cells secreting Th1 (T helper type 1)-cytokines. Taken together, CLIP 3D–printed microneedles coated with vaccine components provide a useful platform for a noninvasive, self-applicable vaccination.
https://www.science.org/doi/10.1126/scitranslmed.aay7162
A pattern of 1.5-millimeter microneedles that contain vaccine and fluorescent quantum dots are applied as a patch. The needles dissolve under the skin, leaving the encapsulated quantum dots. Their pattern can be read to identify the vaccine that was administered. The project was co-led by Rice University bioengineer Kevin McHugh during his time at MIT.
Accurate medical recordkeeping is a major challenge in many low-resource settings where well-maintained centralized databases do not exist, contributing to 1.5 million vaccine-preventable deaths annually. Here, we present an approach to encode medical history on a patient using the spatial distribution of biocompatible, near-infrared quantum dots (NIR QDs) in the dermis. QDs are invisible to the naked eye yet detectable when exposed to NIR light. QDs with a copper indium selenide core and aluminum-doped zinc sulfide shell were tuned to emit in the NIR spectrum by controlling stoichiometry and shelling time. The formulation showing the greatest resistance to photobleaching after simulated sunlight exposure (5-year equivalence) through pigmented human skin was encapsulated in microparticles for use in vivo. In parallel, microneedle geometry was optimized in silico and validated ex vivo using porcine and synthetic human skin. QD-containing microparticles were then embedded in dissolvable microneedles and administered to rats with or without a vaccine. Longitudinal in vivo imaging using a smartphone adapted to detect NIR light demonstrated that microneedle-delivered QD patterns remained bright and could be accurately identified using a machine learning algorithm 9 months after application. In addition, codelivery with inactivated poliovirus vaccine produced neutralizing antibody titers above the threshold considered protective. These findings suggest that intradermal QDs can be used to reliably encode information and can be delivered with a vaccine, which may be particularly valuable in the developing world and open up new avenues for decentralized data storage and biosensing.
“The Bill and Melinda Gates Foundation came to us and said, ‘Hey, we have a real problem — knowing who’s vaccinated,’” said McHugh, who was recruited to join Rice with funding from the Cancer Prevention and Research Institute of Texas. “They said, ‘We go on vaccination campaigns where people get into Hummers, drive to a rural village, set up a tent and start immunizing people, but they don’t always know who’s been immunized before and what vaccines are still needed.”
Parents often don’t know their children’s vaccination histories, McHugh said. “So our idea was to put the record on the person,” he said. “This way, later on, people can scan over the area to see what vaccines have been administered and give only the ones still needed.
The square-centimeter patches hold up to 16 tiny needles. “They don’t go very deep, which makes them theoretically painless and a lot easier for kids,” McHugh said. “They’re like putting on a bandage.”
>microneedles that contain vaccine and fluorescent quantum dots
I wonder what they'll use
https://en.wikipedia.org/wiki/Luciferase
>microneedles that contain vaccine and fluorescent quantum dots
https://www.science.org/doi/suppl/10.1126/scitranslmed.aay7162/suppl_file/aay7162_sm.pdf
https://www.science.org/doi/10.1126/scitranslmed.aay7162
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7532118/
QDs with a copper indium selenide core and aluminum-doped zinc sulfide shell were tuned to emit in the NIR spectrum by controlling stoichiometry and shelling time.
charging laser
>Schwab also endorses "active implantable microchips
https://twitter.com/iamanja123/status/1441543647061753856
https://www.bbc.com/news/uk-england-manchester-58685035
Ex-Rochdale footballer jailed for 24 years for child rapes
A footballer has been jailed for 24 years for "gruesome" sex attacks on six victims as young as seven.
Jonathan Diba-Musangu, 23, who used to be on Rochdale AFC's books, raped the seven-year-old girl in 2012.
The Crown Prosecution Service (CPS) also said he raped a 12-year-old girl at a summer camp four years later.
He was jailed at Manchester Minshull Street Crown Court for 12 offences including three counts of rape of a child under 13 and two counts of rape.
Diba-Musangu, of Fernhurst Street, Chadderton, was also convicted of two counts of sexually assaulting a child under 13, two counts of inciting a child to engage in sexual activity and three counts of sexual activity with a child.
Greater Manchester Police (GMP) said he met most of his victims through a church in Rochdale where he taught Sunday school and music classes.
>Charles Lieber
https://en.wikipedia.org/wiki/Thousand_Talents_Plan
Overseas High-Level Talent Recruitment Programs
established in 2008 by the central government of China to recognize and recruit leading international experts in scientific research, innovation, and entrepreneurship. Both the United States and Canada have warned that China intends to use scientists who are involved with this plan to gain access to new technology for economic and military advantage.
The program grew out of the "Talent Superpower Strategy" of the 17th National Congress of the Chinese Communist Party in 2007. The Central Committee of the Chinese Communist Party and State Council of the People's Republic of China elevated the program in 2010 to become the top-level award given through China's National Talent Development Plan to strengthen innovation and international competitiveness within China. In 2019, the program was re-branded as the "National High-end Foreign Experts Recruitment Plan." The United Front Work Department's Western Returned Scholars Association is the official representative body for program participants.
1000 Talent Plan professorship is the highest academic honor awarded by the State Council, analogous to the top-level award given by the Ministry of Education. The program includes two mechanisms: resources for permanent recruitment into Chinese academia, and resources for short-term appointments that typically target international experts who have full-time employment at a leading international university or research laboratory.
>What woke you the most from your sleep state?
the way the media was playing with the Syrian war
>It's interesting.
https://www.justice.gov/opa/pr/huawei-cfo-wanzhou-meng-admits-misleading-global-financial-institution
Huawei CFO Wanzhou Meng Admits to Misleading Global Financial Institution
Meng Enters into Deferred Prosecution Agreement to Resolve Fraud Charges
The Chief Financial Officer of Huawei Technologies Co. Ltd., Wanzhou Meng, 49, of the People’s Republic of China (PRC), appeared today in federal district court in Brooklyn, entered into a deferred prosecution agreement (DPA) and was arraigned on charges of conspiracy to commit bank fraud and conspiracy to commit wire fraud, bank fraud and wire fraud.
“In entering into the deferred prosecution agreement, Meng has taken responsibility for her principal role in perpetrating a scheme to defraud a global financial institution,” said Acting U.S. Attorney Nicole Boeckmann for the Eastern District of New York. “Her admissions in the statement of facts confirm that, while acting as the Chief Financial Officer for Huawei, Meng made multiple material misrepresentations to a senior executive of a financial institution regarding Huawei’s business operations in Iran in an effort to preserve Huawei’s banking relationship with the financial institution. The truth about Huawei’s business in Iran, which Meng concealed, would have been important to the financial institution’s decision to continue its banking relationship with Huawei. Meng’s admissions confirm the crux of the government’s allegations in the prosecution of this financial fraud — that Meng and her fellow Huawei employees engaged in a concerted effort to deceive global financial institutions, the U.S. government and the public about Huawei’s activities in Iran.”
“This Deferred Prosecution Agreement will lead to the end of the ongoing extradition proceedings in Canada, which otherwise could have continued for many months, if not years,” said Acting Assistant Attorney General Mark J. Lesko for the Justice Department’s National Security Division. “We are enormously grateful to Canada’s Department of Justice for its dedicated work on this extradition and for its steadfast adherence to the rule of law.”
“Financial institutions are our first line of defense in maintaining the safety and security of the U.S. financial system,” said Assistant Attorney General Kenneth A. Polite Jr. of the Justice Department’s Criminal Division. “That is why the law requires that companies who avail themselves of the U.S. financial system provide financial institutions with truthful information about their business operations. Meng Wanzhou, CFO of Huawei Technologies, admitted today that she failed to tell the truth about Huawei’s operations in Iran, and as a result the financial institution continued to do business with Huawei in violation of U.S. law. Our prosecution team continues to prepare for trial against Huawei, and we look forward to proving our case against the company in court.”
“Meng's admissions are evidence of a consistent pattern of deception to violate U.S. law,” said Assistant Director Alan E. Kohler Jr. of the FBI’s Counterintelligence Division. “The FBI will continue to aggressively investigate companies doing business in the United States when there are signs they behave with contempt for our laws.”
>someone please transcribe this interview
I get the question quite a bit, um and I would say um, no.
I think if people, people will ask me how we're different and I said well,
We we have hundreds of papers, like I have um, I have a culture of transparent science like
going back to my my my thesis on like choice and transparency like
we are an incredibly science driven company and theranos was never transparent
like it's just it's and to be honest Theranos was a ?
This woman talks retarded.
https://www.nytimes.com/2021/09/20/opinion/23andme-dna.html
Well, they definitely — look, there was definitely change. Because I mean, one thing that people don’t always recognize about 23andMe is that we had actually engaged with the FDA prior to that warning letter. And it was originally, sort of, reiterated. Like, you are not a medical device, and we are not going to regulate this space. And so world changes. We also started getting more into the medical world. And it was really clear. Like, we did not speak the language of the FDA. And you know, what I learned is, it’s like the DMV. When you go to the DMV, you don’t negotiate whether or not you need a vision test. You just do it. And there’s parts of the FDA that you need to have something similar. Like, there’s a form of obedience. When they tell you that you need to do something, you just need to do it. And I think what I’ve learned is, they do have a global picture that is bigger than what I can see. So one, there’s a lot of respect for the fact that there’s a lot of bad actors that are out there generally. Second is that they do have a job of protecting public safety, and the onus is on us to prove out that something is safe. I find that the FDA can be quite rational when you’re speaking in data. And so if you have a new idea, it just has to be proven out. So I think that’s where it’s really coming up with a reasonable plan that is going to prove out that something actually works. And there’s plenty of things — again, we’ve all seen the bad actors. Like, there’s definitely bad actors that come.
>And I think what I’ve learned is, they do have a global picture that is bigger than what I can see.
https://en.wikipedia.org/wiki/Susan_Del_Percio
https://archive.nytimes.com/www.nytimes.com/learning/general/onthisday/big/0901.html
"At 8 P.M., according to the semi-official news agency, a group of Polish insurrectionists forced an entrance into the Gleiwitz radio station [in Germany], overpowering the watchmen and beating and generally mishandling the attendants. The Gleiwitz station was relaying a Breslau station’s program, which was broken off by the Poles."
>"At 8 P.M., according to the semi-official news agency, a group of Polish insurrectionists forced an entrance into the Gleiwitz radio station [in Germany], overpowering the watchmen and beating and generally mishandling the attendants. The Gleiwitz station was relaying a Breslau station’s program, which was broken off by the Poles."
https://en.wikipedia.org/wiki/Gleiwitz_incident
The Gleiwitz incident was a false flag attack on the German radio station Sender Gleiwitz, staged by Nazi Germany on the night of 31 August 1939. Along with some two dozen similar incidents, the attack was manufactured by Germany as a casus belli to justify the invasion of Poland, which began the next morning. The attackers posed as Polish nationals.
>The Gleiwitz incident was a false flag attack on the German radio station Sender Gleiwitz, staged by Nazi Germany on the night of 31 August 1939. Along with some two dozen similar incidents, the attack was manufactured by Germany as a casus belli to justify the invasion of Poland, which began the next morning. The attackers posed as Polish nationals.
Much of what is known about the Gleiwitz incident comes from the affidavit of SS-Sturmbannführer Alfred Naujocks at the Nuremberg Trials. In his testimony, he stated that he organised the incident under orders from Reinhard Heydrich and Heinrich Müller, chief of the Gestapo. On the night of 31 August, a small group of German operatives dressed in Polish uniforms and led by Naujocks seized the Gleiwitz station and broadcast a short anti-German message in Polish (sources vary on the content of the message). The operation was named "Grossmutter gestorben" (Grandmother died). The operation was to make the attack and the broadcast look like the work of Polish anti-German saboteurs.
To make the attack seem more convincing, the Gestapo murdered Franciszek Honiok, a 43-year-old unmarried German Silesian Catholic farmer, known for sympathising with the Poles. He had been arrested the previous day by the Gestapo and dressed to look like a saboteur, then killed by lethal injection and given gunshot wounds. Honiok was left dead at the scene so that he appeared to have been killed while attacking the station. His corpse was then presented to the police and press as proof of the attack. Several prisoners from the Dachau concentration camp were drugged, shot dead on the site and their faces disfigured to make identification impossible. The Germans referred to them by the code phrase "Konserve" (canned goods). Some sources incorrectly refer to the incident as Operation Canned Goods.
In an oral testimony at the trials, Erwin von Lahousen stated that his division of the Abwehr was one of two that were given the task of providing Polish Army uniforms, equipment and identification cards; he was later told by Wilhelm Canaris that people from concentration camps had been disguised in these uniforms and ordered to attack the radio stations.
Oskar Schindler who later is credited with saving the lives of 1,200 Jews during the Holocaust, played a role in supplying the Polish uniforms and weapons used in the operation as an agent for the Abwehr.
>Oskar Schindler who later is credited with saving the lives of 1,200 Jews during the Holocaust, played a role in supplying the Polish uniforms and weapons used in the operation as an agent for the Abwehr.
https://www.timesofisrael.com/80-years-ago-how-a-very-different-schindlers-list-helped-ignite-wwii/
80 years ago, how a very different Schindler’s ‘list’ helped ignite WWII
Controlling up to two dozen German spies, Oskar Schindler helped stage the false-flag ‘Gleiwitz Incident’ to justify Germany’s invasion of Poland
Oskar Schindler shielded 1,200 Jewish factory workers during the Holocaust and a Hollywood movie about the heroic rescue has turned his name into the archetype for a Righteous Among the Nations. However, the Nazi-turned-savior also played less altruistic roles during Hitler’s rule.
Ignored by the book and film bearing his name, Schindler helped Germany lay groundwork for its invasion of Poland 80 years ago. Leading up to the blitzkrieg attack launched on September 1, 1939, Schindler led a network of 25 spies and prepared an infamous false-flag attack called the “Gleiwitz Incident.”
By all accounts, Schindler during the mid-30s was “unemployed and frustrated,” not to mention an alcoholic who owed many debts. He racked up criminal charges and prison stints, including for brawling, dangerous threats, and swindling. Schindler’s failed gigs ranged from poultry farming to banking, and he was known as “Schindler Swindler.”
During a series of high-society parties, Schindler impressed the right Nazi spy masters. One of them recruited him into Germany’s Abwehr (military intelligence), and Schindler was sent to Ostrava in today’s Czech Republic. Located near the border with Poland, the city was a hotbed of agents and double-agents.
Contrary to the suave manipulator portrayed in the film “Schindler’s List,” the 30 year old’s first stint as a spy was comical. People in Ostrava knew Schindler by name and he did not bother to change his address, all while gathering information on Czech military defenses. This carelessness led to Schindler’s arrest with the charge of capital offenses against the Czech state.
>Controlling up to two dozen German spies, Oskar Schindler helped stage the false-flag ‘Gleiwitz Incident’ to justify Germany’s invasion of Poland
Contrary to the film portrayal of Schindler entering Krakow on the heels of the German army, he started eyeing businesses and homes there before the invasion of Poland. During the period that Schindler was getting to know Krakow, his agents were gathering supplies for the coming false-flag attack at Gleiwitz — Polish army uniforms? Check. Weapons? Check. Polish cigarettes? Check.
>I just wish an Historian with integrity could make up their mind on this.
I think that's us
>the only transparency is her own nervous system exposing her bs
https://www.youtube.com/watch?v=b62jCtUJ8Fc
50:20
can someone throw that on streamable
https://www.youtube.com/watch?v=b62jCtUJ8Fc&t=3385s