Anonymous ID: 52c259 Oct. 6, 2021, 5:54 a.m. No.14731997   🗄️.is 🔗kun   >>2014

“This is no small thing, to restore a republic after it has fallen into corruption. I have studied history for years and I cannot recall it ever happening. It may be that our task is impossible. Yet, if we do not try then how will we know it can’t be done? And if we do not try, it most certainly won’t be done. The Founders’ Republic, and the larger war for western civilization, will be lost.”

 

“But I tell you this: We will not go gently into that bloody collectivist good night. Indeed, we will make with our defiance such a sound as ALL history from that day forward will be forced to note, even if they despise us in the writing of it.”

Anonymous ID: 52c259 Oct. 6, 2021, 7:03 a.m. No.14732279   🗄️.is 🔗kun   >>2286 >>2609

https://www.cnn.com/2020/12/09/politics/hunter-biden-tax-investigtation/index.html

Federal criminal investigation into Hunter Biden focuses on his business dealings in China

"I learned yesterday for the first time that the U.S. Attorney's Office in Delaware advised my legal counsel, also yesterday, that they are investigating my tax affairs. I take this matter very seriously but I am confident that a professional and objective review of these matters will demonstrate that I handled my affairs legally and appropriately, including with the benefit of professional tax advisors," Hunter Biden said in a statement.

Anonymous ID: 52c259 Oct. 6, 2021, 7:09 a.m. No.14732307   🗄️.is 🔗kun   >>2314 >>2317 >>2342 >>2440 >>2542 >>2680

https://www.theguardian.com/science/neurophilosophy/2016/mar/24/magneto-remotely-controls-brain-and-behaviour

Genetically engineered 'Magneto' protein remotely controls brain and behaviour

“Badass” new method uses a magnetised protein to activate brain cells rapidly, reversibly, and non-invasively

Researchers in the United States have developed a new method for controlling the brain circuits associated with complex animal behaviours, using genetic engineering to create a magnetised protein that activates specific groups of nerve cells from a distance.

Understanding how the brain generates behaviour is one of the ultimate goals of neuroscience – and one of its most difficult questions. In recent years, researchers have developed a number of methods that enable them to remotely control specified groups of neurons and to probe the workings of neuronal circuits.

The most powerful of these is a method called optogenetics, which enables researchers to switch populations of related neurons on or off on a millisecond-by-millisecond timescale with pulses of laser light. Another recently developed method, called chemogenetics, uses engineered proteins that are activated by designer drugs and can be targeted to specific cell types.

Although powerful, both of these methods have drawbacks. Optogenetics is invasive, requiring insertion of optical fibres that deliver the light pulses into the brain and, furthermore, the extent to which the light penetrates the dense brain tissue is severely limited. Chemogenetic approaches overcome both of these limitations, but typically induce biochemical reactions that take several seconds to activate nerve cells.

The new technique, developed in Ali Güler’s lab at the University of Virginia in Charlottesville, and described in an advance online publication in the journal Nature Neuroscience, is not only non-invasive, but can also activate neurons rapidly and reversibly.

Several earlier studies have shown that nerve cell proteins which are activated by heat and mechanical pressure can be genetically engineered so that they become sensitive to radio waves and magnetic fields, by attaching them to an iron-storing protein called ferritin, or to inorganic paramagnetic particles. These methods represent an important advance – they have, for example, already been used to regulate blood glucose levels in mice – but involve multiple components which have to be introduced separately.

The new technique builds on this earlier work, and is based on a protein called TRPV4, which is sensitive to both temperature and stretching forces. These stimuli open its central pore, allowing electrical current to flow through the cell membrane; this evokes nervous impulses that travel into the spinal cord and then up to the brain.

Güler and his colleagues reasoned that magnetic torque (or rotating) forces might activate TRPV4 by tugging open its central pore, and so they used genetic engineering to fuse the protein to the paramagnetic region of ferritin, together with short DNA sequences that signal cells to transport proteins to the nerve cell membrane and insert them into it.

Anonymous ID: 52c259 Oct. 6, 2021, 7:11 a.m. No.14732314   🗄️.is 🔗kun   >>2319 >>2325

>>14732307

>https://www.theguardian.com/science/neurophilosophy/2016/mar/24/magneto-remotely-controls-brain-and-behaviour

When they introduced this genetic construct into human embryonic kidney cells growing in Petri dishes, the cells synthesized the ‘Magneto’ protein and inserted it into their membrane. Application of a magnetic field activated the engineered TRPV1 protein, as evidenced by transient increases in calcium ion concentration within the cells, which were detected with a fluorescence microscope.

Next, the researchers inserted the Magneto DNA sequence into the genome of a virus, together with the gene encoding green fluorescent protein, and regulatory DNA sequences that cause the construct to be expressed only in specified types of neurons. They then injected the virus into the brains of mice, targeting the entorhinal cortex, and dissected the animals’ brains to identify the cells that emitted green fluorescence. Using microelectrodes, they then showed that applying a magnetic field to the brain slices activated Magneto so that the cells produce nervous impulses.

To determine whether Magneto can be used to manipulate neuronal activity in live animals, they injected Magneto into zebrafish larvae, targeting neurons in the trunk and tail that normally control an escape response. They then placed the zebrafish larvae into a specially-built magnetised aquarium, and found that exposure to a magnetic field induced coiling manouvres similar to those that occur during the escape response. (This experiment involved a total of nine zebrafish larvae, and subsequent analyses revealed that each larva contained about 5 neurons expressing Magneto.)

In one final experiment, the researchers injected Magneto into the striatum of freely behaving mice, a deep brain structure containing dopamine-producing neurons that are involved in reward and motivation, and then placed the animals into an apparatus split into magnetised a non-magnetised sections. Mice expressing Magneto spent far more time in the magnetised areas than mice that did not, because activation of the protein caused the striatal neurons expressing it to release dopamine, so that the mice found being in those areas rewarding. This shows that Magneto can remotely control the firing of neurons deep within the brain, and also control complex behaviours.

Neuroscientist Steve Ramirez of Harvard University, who uses optogenetics to manipulate memories in the brains of mice, says the study is “badass”.

“Previous attempts [using magnets to control neuronal activity] needed multiple components for the system to work – injecting magnetic particles, injecting a virus that expresses a heat-sensitive channel, [or] head-fixing the animal so that a coil could induce changes in magnetism,” he explains. “The problem with having a multi-component system is that there’s so much room for each individual piece to break down.”

“This system is a single, elegant virus that can be injected anywhere in the brain, which makes it technically easier and less likely for moving bells and whistles to break down,” he adds, “and their behavioral equipment was cleverly designed to contain magnets where appropriate so that the animals could be freely moving around.”

‘Magnetogenetics’ is therefore an important addition to neuroscientists’ tool box, which will undoubtedly be developed further, and provide researchers with new ways of studying brain development and function.

Reference

Wheeler, M. A., et al. (2016). Genetically targeted magnetic control of the nervous system. Nat. Neurosci., DOI: 10.1038/nn.4265 [Abstract]

Anonymous ID: 52c259 Oct. 6, 2021, 7:23 a.m. No.14732382   🗄️.is 🔗kun   >>2386 >>2390 >>2392 >>2412

https://www.dailymail.co.uk/news/article-10063129/ESPN-anchor-Sage-Steele-taken-air-controversial-comments-recovers-COVID-19.html

Bi-racial ESPN anchor Sage Steele is taken off air after questioning why Obama identifies as black when 'he was raised by white mom', calling vaccine mandates 'sick' and saying female reporters 'know what they doing' when they wear sexy outfits

 

Sage Steele has taken a break from TV after she made controversial comments about race, sexism and coronavirus protocols during interview with Jay Cutler

Steele called ESPN's vaccine mandate 'sick,' commented on Obama'a blackness and accused female journalists of welcoming harassment

She was placed on a break and will not take part in the network's espnW summit, which focuses on women in sports

Her break was attributed to her controversial comments, however a source familiar with the situation also claims Steele tested positive for COVID

She is expected to return to full duty at ESPN next week

Anonymous ID: 52c259 Oct. 6, 2021, 7:29 a.m. No.14732404   🗄️.is 🔗kun   >>2407

https://www.usatoday.com/story/news/nation/2021/10/05/ohio-sex-trafficking-sting-nets-161-arrests-elyria-councilman/6008063001/

Largest human trafficking sting in Ohio history nets 161, including city councilman

A firefighter, college professor and a Cleveland-area city councilman were among 161 people arrested in a sex sting operation last week, described as the state's largest focused on human trafficking.

Ohio Attorney General Dave Yost, law enforcement officials from multiple counties, social service providers and former human trafficking victims met in the Ohio Statehouse on Monday to talk about the initiative.

Dubbed "Operation Ohio Knows," the weeklong sting was intended to create a deterrent for those who seek sex for pay or profit, Yost said.

"We want to send a message to everybody in the country: Don't buy sex in Ohio," he said.

'Operation Ohio Knows'

Law enforcement fanned out across the state, communicating with hundreds of men seeking sex. For every arrest made, officers contacted up to eight "johns" whose actions didn't meet the elements of any crime, said Chief Deputy Rick Minerd of the Franklin County sheriff's office.

Three of the perpetrators were willing to pay to have sex with people they thought were minors. Ten minors reported missing were recovered during a simultaneous operation carried out by the U.S. Marshals Service.

Fifty-one women, would-be victims, were provided assistance by social service advocates. It was unclear how many of the women were charged.

Most of those arrested were charged with engaging in prostitution, a first-degree misdemeanor. Other charges related to drugs and firearms.

A change in state law passed in the spring requires those convicted to undergo human trafficking education.

"We cannot arrest our way out of human trafficking," Yost said, noting that arrests are nevertheless important as a deterrence. "If there are no buyers, there will be no trafficking."

He wants people to know that it's not a victimless crime.

"When you are the buyer, you have no idea who you're dealing with," he said. "The pimp, the trafficker, doesn't show up and sit in the corner watching you. The survivor doesn't tell you, 'I don't want to do this, but if I don't, I'm going to be beat' or 'He's going to withhold my drugs.'

"Because we don't know, anybody in Ohio who purchases sex is assuming the risk that they're complicit in trafficking."

Human trafficking survivor Mandie Knight spoke via Zoom about her former lifestyle and her gratitude at being arrested.

“Had I not been arrested, had I not gone to jail and had I not suffered some consequences for the decisions I was making, I wouldn’t be here today, and I wouldn’t be as successful in life," said Knight, a wife, mother and student in forensic criminology.

Anonymous ID: 52c259 Oct. 6, 2021, 7:30 a.m. No.14732407   🗄️.is 🔗kun   >>2440 >>2542 >>2625 >>2680

>>14732404

>Largest human trafficking sting in Ohio history nets 161, including city councilman

Arrested city councilman: 'An enormous mistake'

Mark Jessie, a councilman running for reelection Nov. 2 in Elyria, Ohio, a city roughly 30 miles outside Cleveland, was picked up in the sting.

"I'm taking this very seriously and realize it's an enormous mistake," he told The Columbus Dispatch, part of the USA TODAY Network, on Monday. Jessie said he sought sex from someone he thought was selling it on a website.

When officers arrested him, he said, "there was an immediate feeling of wanting to throw up and an immediate feeling of facing the consequences with my family and friends."

Summit County Sheriff Kandy Fatheree cautioned her colleagues not to rest on sting operations alone.

"This is the beginning and not the end, and I think we have so much work to do across the state," she said.

Anonymous ID: 52c259 Oct. 6, 2021, 7:31 a.m. No.14732414   🗄️.is 🔗kun   >>2443 >>2463

https://www.dailymail.co.uk/news/article-10062775/US-Chinese-scientists-planning-create-brand-new-coronavirus-leaked-proposals-show.html

Wuhan scientists and US researchers planned to create a new coronavirus in 2018: Consortium led by Brit Peter Daszak asked DARPA to fund research at lab in city where Covid pandemic began

 

A 2018 grant proposal sought to combine data from similar strains for new virus

It was submitted by scientists from US, China and Singapore, but was rejected

A genetics expert from the WHO told The Telegraph that such work could explain why a close ancestor for Covid-19 has yet to be found in nature

The Wuhan Institute of Virology has consistently denied creating Covid-19

Anonymous ID: 52c259 Oct. 6, 2021, 7:35 a.m. No.14732437   🗄️.is 🔗kun

https://twitter.com/AlexThomp/status/1445756210561515532

Kamala Harris has a YouTube Kids original coming out tomorrow for Space Week that highlights her role as head of the space council.

Anonymous ID: 52c259 Oct. 6, 2021, 7:40 a.m. No.14732455   🗄️.is 🔗kun   >>2466

https://www.theguardian.com/us-news/2021/jan/27/proud-boys-leader-enrique-tarrio-fbi-informant

Proud Boys leader Enrique Tarrio was an FBI informant

Extremist leader repeatedly worked undercover for investigators after his arrest in 2012, former prosecutor and court files reveal

Enrique Tarrio, the leader of the Proud Boys extremist group, has a past as an informer for federal and local law enforcement, repeatedly working undercover for investigators after he was arrested in 2012, according to a former prosecutor and a transcript of a 2014 federal court proceeding obtained by Reuters.

In the Miami hearing, a federal prosecutor, a Federal Bureau of Investigation agent and Tarrio’s own lawyer described his undercover work and said he had helped authorities prosecute more than a dozen people in various cases involving drugs, gambling and human smuggling.