Anonymous ID: 5aee5c Oct. 9, 2021, 6:42 p.m. No.14756530   🗄️.is 🔗kun   >>6551 >>6561 >>6579 >>6602 >>6612 >>6624 >>6647

1/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

What is HEK 293 and where did it come from? News Medical Life Sciences provides some answers in the article below.

"HEK293 Cells: Applications and Advantages"

 

"TheHEK 293 cell linewas initially produced in1973by a team led by Alex van der Eb in Leiden (Netherlands) from normal fetal human embryonic kidney cells."

 

"Human embryonic kidney 293 (HEK293, HEK-293, or HEK) cells are one of the most common cell lines used for research purposes, second only to HeLa cells. Their popularity is due to several properties such as easy maintenance, robustness, andease of transfection."

https://www.news-medical.net/life-sciences/HEK293-Cells-Applications-and-Advantages.aspx

 

Transfection defined (think RNA and DNA).

The Free Dictionary

https://medical-dictionary.thefreedictionary.com/transfection

Anonymous ID: 5aee5c Oct. 9, 2021, 6:44 p.m. No.14756551   🗄️.is 🔗kun   >>6647

>>14756530

2/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

The Federal RePORTER lists 180 funded projects involving HEK 293 cells. To see the list go to

https://federalreporter.nih.gov/

and type HEK 293 into the search bar.

 

Justia Patents lists a 2004 patent #6984521, held by the Korea Institute of Science and Technology, involving

"HEK293 cell line for activity studies and HTS system of αT-type Ca channel."

May 28, 2004 - Korea Institute of Science and Technology

https://patents.justia.com/patent/6984521

Anonymous ID: 5aee5c Oct. 9, 2021, 6:46 p.m. No.14756561   🗄️.is 🔗kun   >>6594 >>6647

>>14756530

3/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

An outfit called ABCAM apparently sells HEK 293 cell lines.

Go to

https://www.abcam.com/products

Type inHEK 293and get1642 results.

 

Anon found it interesting that Prion Protein (PrP) and HEK 293 cells are linked in this ABCAM offering. It's also interesting that alternative names for this offering includeCJDandKuruand the storage temperature must be between-4 and -112 degrees Fahrenheit. Sound familiar?

 

"Recombinant Human Prion protein PrP (Fc Chimera) (ab276456)"

 

"NotePrPis found in high quantity in the brain of humans and animals infected with neurodegenerative diseases known as transmissible spongiform encephalopathies or prion diseases, like: Creutzfeldt-Jakob disease (CJD), fatal familial insomnia (FFI), Gerstmann-Straussler disease (GSD), Huntington disease-like type 1 (HDL1) and kuru in humans; scrapie in sheep and goat; bovine spongiform encephalopathy (BSE) in cattle; transmissible mink encephalopathy (TME); chronic wasting disease (CWD) of mule deer and elk; feline spongiform encephalopathy (FSE) in cats and exotic ungulate encephalopathy (EUE) in nyala and greater kudu. The prion diseases illustrate three manifestations of CNS degeneration: (1) infectious (2) sporadic and (3) dominantly inherited forms. TME, CWD, BSE, FSE, EUE are all thought to occur after consumption of prion-infected foodstuffs."

 

https://www.abcam.com/recombinant-human-prion-protein-prp-fc-chimera-ab276456.html

Anonymous ID: 5aee5c Oct. 9, 2021, 6:48 p.m. No.14756579   🗄️.is 🔗kun   >>6590 >>6647

>>14756530

4/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

Here are more ABCAM Prion Protein (PrP) offerings followed by sauce for Merriam-Webster's definition of Prion Protein.

 

Recombinant Mouse Prion protein PrP (ab74056)

https://www.abcam.com/recombinant-mouse-prion-protein-prp-ab74056.html

 

Recombinant Cow Prion protein PrP (ab753)

https://www.abcam.com/recombinant-cow-prion-protein-prp-ab753.html

 

Recombinant Cow Prion protein PrP (mutated F209S) (ab90587)

https://www.abcam.com/recombinant-cow-prion-protein-prp-mutated-f209s-ab90587.html

 

Recombinant Human Prion protein PrP (denatured) (ab140567)

https://www.abcam.com/recombinant-human-prion-protein-prp-denatured-ab140567.html?productWallTab=ShowAll

 

Recombinant Sheep Prion protein PrP (ab74200)

https://www.abcam.com/recombinant-sheep-prion-protein-prp-ab74200.html

 

Prion ProteinDefined

https://www.merriam-webster.com/dictionary/prion

Anonymous ID: 5aee5c Oct. 9, 2021, 6:49 p.m. No.14756602   🗄️.is 🔗kun   >>6647

>>14756530

5/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

ABCAM Board

https://corporate.abcam.com/investors/boardandleadership/

 

Shareholders

https://corporate.abcam.com/investors/major-shareholders/

 

Advisers

ABCAM's corporate headquarters are atCambridge Biomedical Campus, Cambridge, UK.

https://corporate.abcam.com/investors/advisers/

 

ABCAM - Our People

https://corporate.abcam.com/about/our-people/

 

Our Markets

https://corporate.abcam.com/investors/our-markets/

 

ABCAM - About

"We are a global life science company supporting customers at the forefront of life science research. Our innovative products are used by hundreds of thousands of scientists worldwide."

https://corporate.abcam.com/about/

 

ABCAM additional Locations

Offices inUS, China, Hong Kong and Japan.

https://craft.co/abcam/locations

 

www.abcam.cn

研究用途的产品诊断和治疗的解决方案技术支持活动通知通路

https://www.abcam.cn/

 

Your breakthrough starts here

キット、試薬、抗体、… 次なるディスカバリーのために

https://www.abcam.co.jp/

Anonymous ID: 5aee5c Oct. 9, 2021, 6:50 p.m. No.14756612   🗄️.is 🔗kun   >>6647

>>14756530

6/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

This Plos One article also links HEK 293 Cells with protein. It discusses the temperatures at which recombinant proteins are most productive and much, much more that MedAnons can hopefully interpret.

 

"Enhancing Protein Expression in HEK-293 Cells by Lowering Culture Temperature"

"By following the time course of the GFP-expressing cells growing at37°C and 33°Cfrom 24 hours aftertransfection(including 19 hours recovery at 37°C in the normal growth medium), we found that amild hypothermia(i.e., 33°C)reduces the growth rateof HEK-293S cells,

while increasing cellular productivity of recombinant proteins."

The article is very long. Find much more here v v v.

https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0123562

Anonymous ID: 5aee5c Oct. 9, 2021, 6:51 p.m. No.14756624   🗄️.is 🔗kun   >>6647

>>14756530

7/7

HEK 293 Dig, ABCAM, Recombinant Human Prion protein PrP, NIH and a Dead Baby

 

Anons may wonder what scientific purpose the recombinant proteins serve. This Cusabio article describes their use in medicine, research, and biotechnology. It also discuses some of the latest recombinant protein projects.

 

Applications of recombinant proteins

https://www.cusabio.com/c-20272.html#:~:text=Applications%20of%20recombinant%20proteins%201%20Medicine.%20Most%20human,…%204%20The%20Latest%20Researches%20of%20Recombinant%20Proteins