This dig is huge. IRL is distracting Anon from doing it justice. Calling for help on this one. Will include as much sauce as possible. IRL for the rest of the week but really think this is important.

Conflict of interest much?

1/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

All PB

>>14875343, >>14875353, >>14875363, >>14875384 Eric Lander, and Much More, Needs Digs

Anons rememberEric Landerfrom previous digs. He's currently thePresident’sScience Advisor andDirector of the White HouseOffice of Science and TechnologyPolicy(OSTP). He's also afounding director of the Broad Institute(think Harvard and MIT). Broad Institute holds 166 patents, at least 36 of which deal with CRISPR Technology. Feng Zhang (Broad Institute, MIT) is the guy who pioneered the CRISPR-Cas9 technology. Something called Addgene was spawned by the Broad Institute. "Addgene is a nonprofit plasmid repository dedicated to improving life science research." Scientists can submit their discoveries and the repository is dedicated tosharingthose discoveries World Wide. Also Spawned by the Broad Institute is something called the Zhang Lab.

The CRISPR-Cas system is the subject of an NCBI NLM NIH review dated May 13, 2021 and titled "COVID-19 one year later: aretrospectof CRISPR-Cas system in combating COVID-19". The review emphasizes the importance ofCRISPR-Cas system gene editing toolin diagnosis and treatment of Covid-19.

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NCBI NLM NIH

"COVID-19 one year later: a retrospect of CRISPR-Cas system in combating COVID-19"

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8193275/

Retrospectdefined.

Retrospect means a review of past events.

https://www.merriam-webster.com/dictionary/retrospect

Found166 patents, related to the Broad Institute, in the US Patent Database. At least thirty-six patents, in this search, are related toCRISPRin some way.

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Go to US Patent Database quick search

https://patft1.uspto.gov/netahtml/PTO/search-bool.html

type Broad Institute into "Term 1" and select Assignee Name in the "Field" search bar. Get 166 results.

>>14912914

2/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

CRISPR technology had an upgrade in 2015. It's called the CPF1 system.

"Scientists discover new system for human genome editing with potential to increase power and precision of genome engineering" The Sept. 25, 2015 Broad Institute press release shares a lot of information about who is involved in the development of CRISPR technology and gene editing. Anons will also find the picture interesting.

https://www.broadinstitute.org/news/scientists-discover-new-system-human-genome-editing-potential-increase-power-and-precision

"A team including the scientist who first harnessed the revolutionary CRISPR-Cas9 system for mammalian genome editing has now

identified a different CRISPR systemwith the potential for evensimpler and more precisegenome engineering."

"'This has dramatic potential to advance genetic engineering,' saidEric Lander, Director of the Broad Institute

and one of theprincipal leaders of the human genome project. 'The paper not only reveals the function of a

previously uncharacterized CRISPR system, but also shows that Cpf1 can be harnessed forhuman genome editing

and has remarkable and powerful features. The Cpf1 system represents anew generationof genome editing technology.'"

In This Article

CRISPR-Cas9 system for mammalian genome editing

Feng Zhang

Broad Institute -MIT and Harvard-McGovern Institutefor Brain Research at MIT and MIT Department of Biology

Eugene Koonin at the National Institutes of Health

Aviv Regev of the Broad Institute

John van der Oost -Wageningen University

human genome project

mammalian genome editing was first reported in 2013, by Zhang - separately byGeorge Church at Harvard

Cpf1 system -“We are committed to making the CRISPR-Cpf1 technology widely accessible,” Zhang said.

Cpf1 enzymes from bacterial speciesAcidaminococcus and Lachnospiraceae

completely different CRISPR enzymes

business and intellectual property

• First:

• • DNA-cutting enzyme Cas9 forms a complex with two small RNAs, both of which are required for the cutting activity

• • Cpf1 system is simpler in that it requires only a single RNA

• • Cpf1 enzyme is also smaller than the standard SpCas9, making iteasier to deliver into cells and tissues

• Second:

• • Cpf1 cuts DNA in a different manner than Cas9

• • Cas9 complex cuts DNA, it cuts both strands at the same place, leaving ‘blunt ends’ that often undergo mutations

• • Cpf1 complex the cuts in the two strands are offset, leaving short overhangs on the exposed ends

• • help with precise insertion… integrate a piece of DNA more efficiently and accurately

• Third:

• • Cpf1 cuts far away from the recognition site

• • even if the targeted gene becomes mutated at the cut site, it can likely still be re-cut

• • multiple opportunities for correct editing to occur

• Fourth:

• • Cpf1 system provides new flexibility in choosing target sites. Like Cas9

• • first attach to a short sequence known as a PAM

• • targets must be chosen that are adjacent to naturally occurring PAM sequences

• • Cpf1 complex recognizes very different PAM sequences from those of Cas9

• • an advantage in targeting some genomes… malaria parasite as well as in humans

Levi Garraway- a Broad Institute member

Joint Center for Cancer Precision Medicine

Dana-Farber Cancer Institute, Brigham and Women’s Hospital, and the Broad Institute

plan to share the Cpf1 system widely… ensuring availability of this new enzyme to empower research

As with Cas9 -freely available for academic research

plasmid-sharing-websiteAddgene

Zhang lab has already shared Cas9 reagents more than 23,000 times to researchers worldwide(in 2015)

free online tools and resourcesfor researchers through its website…

Our goal is to develop tools that can accelerate research… "We see much more to come…"

>>14912914

3/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

A Federal Reporter search for Eric Lander yields 18 results, at least 6 of which directly involve the Broad Institute. The US taxpayers partially fund the Broad Institute. Anon believes we should be very interested in how the Broad Institute spends money because the CRISPR technologies were pioneered, at the institute, by Feng Zhang under the directorship of Eric Lander.

v v v

Go to Federal RePORTER

https://federalreporter.nih.gov

and enter Eric Lander into search bar.

Four of the 6 Broad Institute projects involve CRISPR technology.

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Federal RePORTER - Project Number 5K99HG009917-02

Project Start Date - 05-January-2018

https://reporter.nih.gov/project-details/9621393

Federal RePORTER - Project Number 1K99HG009917-01

Project Start Date - 05-January-2018

https://reporter.nih.gov/project-details/9431138

Federal RePORTER - Project Number 5K99HG008171-02

Project Start Date - 01-December-2014

https://reporter.nih.gov/project-details/8974432

Federal RePORTER - Project Number 1K99HG008171-01

Project Start Date - 01-December-2014

https://reporter.nih.gov/project-details/8804084

>>14912914

4/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Broad Institute provides a CRISPR timeline which documents CRISPR developments from 1993 through 2013.

"The discovery of the CRISPR-Cas microbial adaptive immune system and its ongoing development into a genome editing tool represents the work of many scientists from around the world."

https://www.broadinstitute.org/what-broad/areas-focus/project-spotlight/crispr-timeline

Broad Institute news and media release says,

"Broad Institute partners with CDC and Theiagen Genomicsto enable pathogen genomic surveillance across the United States".

9-24-21

"The partnership will facilitate the use ofTerra— an open-source platform for biomedical data storage, analysis, and collaboration —in public health labs for genomic surveillance of SARS-CoV-2 and future outbreaks."

https://www.broadinstitute.org/news/broad-institute-partners-cdc-and-theiagen-genomics-enable-pathogen-genomic-surveillance-across

Broad Institute - Founders

Eli and Edythe Broad

https://www.broadinstitute.org/history-leadership/founders

Broad Institute - Board of Directors

Eric E. Schmidt

https://www.broadinstitute.org/people/board-directors

Broad Institute - Board of Scientific Councelors

https://www.broadinstitute.org/people/board-scientific-counselors

Broad Institute,org

https://www.broadinstitute.org/

Broad Institute - Leadership

https://www.broadinstitute.org/people/institute-leadership

Broad Institute - Members

https://www.broadinstitute.org/people/institute-members

Broad Institute - Scientists

https://www.broadinstitute.org/people/institute-scientists

Broad Institute - Program and Platform Directors

https://www.broadinstitute.org/about-us/program-and-platform-directors

Broad Institute - "RESEARCH HIGHLIGHTS: CRISPR"

"The ability to precisely edit the genome of a living cell holds enormous potential…"

For several years prior to 2013, gene editing methods existed but "these were quickly eclipsed by the efficiency, effectiveness and precision of the engineered CRISPR-Cas9 system that was first harnessed for mammalian genome editing byFeng Zhangof the Broad Institute and MIT."

https://www.broadinstitute.org/research-highlights-crispr

Are there any Anons who would like to read a couple of really long articles in something called Cell? Found their logo to be interesting.

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

https://www.cell.com/cell/fulltext/S0092-8674(20)30262-2

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System, Cell (2015)

https://www.cell.com/cell/fulltext/S0092-8674(15)01200-3

>>14912914

5/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Addgene

"A Better Way to Share Science".

"We distribute 104,724 plasmids on behalf of 4,900 labs from around the world. We also produce 705 ready-to-use viral vectors from our plasmid collection. Find what you need for your next experiment."

https://www.addgene.org/

Sponsors and Supporters - Think Chan Zuckerberg Initiative

Anon has 2 questions about the Mexico distributors listed on this Addgene webpage. Who knew there's a San Diego in Mexico? Do they mean San Diego Huehuecalco, Mexico? Digging being the game, Anon researched and found that they do indeed mean San Diego, Ca. Now Anon has another question. Since when has San Diego, CA been in Mexico?

https://www.addgene.org/sponsors/

Addgene distributors page shows that the San Diego/Mexico thing is not a mistake. San Diego, Ca is listed as the distributor in Mexico. Makes Anon wonder if politicians have simply sold parts of the US to foreign interests.

https://help.addgene.org/hc/en-us/articles/206730406-Do-you-accept-orders-from-distributors

Addgene - Leadership (management), Board of Directors, Advisory Board, Alumni

https://www.addgene.org/management/

Addgene - Projects and Partners

https://www.addgene.org/addgene-gives-back/

>>14912914

6/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

A Federal RePORTER search for Addgene produced 27 results- think taxpayer funding.

Go to Federal RePORTER

https://federalreporter.nih.gov

and enter Addgene into search bar, click search.

Addgene - CRISPR Plasmids and Resources

https://www.addgene.org/crispr/

Addgene - Fluorescent Proteins

"Addgene's plasmid repository contains a variety offluorescent protein plasmids. Use this guide to learn more about the many applications offluorescent proteins (FPs)and to find the plasmids that are available from Addgene's depositing scientists."

https://www.addgene.org/fluorescent-proteins/

>>14912914

7/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Addgene - Plasmid Collections

https://www.addgene.org/collections/

Addgene - Repository

https://www.addgene.org/browse/

v v v

Coronavirusfor sale.

Go to above sauce and type corona virus into the search bar. Find 2108 results in the catalog. Click on 2092 Plasmids and find a list of 2092 Plasmids. Anon is confused by this information. Anon wonders if there is a conflict of interest here. Why does Addgene sell coronaviruses and also CRISPR technology to cure Covid-19, which is a coronavirus. Anon requests that science/medical Anons look at this information and let us know what's going on at Addgene. Anon knows that there are thousands of coronaviruses and they may be unrelated to current events but… Anon is IRL distracted and can't find dates to see if Addgene was selling SARS Cov 2 before the pandemic.

Here's one to try, if you like, go to Addgene - https://www.addgene.org/ and typeWuhaninto the search bar. This Anon got 103 results. Click on 102 Plasmids to see the catalog entries.

>>14912914

8/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Plasmid Distribution to Industry - thinkResolute

https://www.addgene.org/industry/

Resolute Consortium at Addgene

https://www.addgene.org/RESOLUTE_Consortium/

Resolute Consortium - re-solute.eu

"RESOLUTE is a public-private partnership with 13 partners from academia and industry with an overarching goal: To trigger an escalation in the appreciation and intensity of research on solute carriers (SLCs) worldwide and to establish SLCs as a tractable target class for medical research and development."

https://re-solute.eu/

Resolute - About

https://re-solute.eu/about

Resolute - Partners - thinkPfizer

https://re-solute.eu/consortium

Resolution

"The new REsolution consortium is a public-private research partnership, supported by the Innovative Medicines Initiative (IMI), with nine partners from academia and the pharmaceutical industry. Starting on June 1, 2021 and with a duration of 2 years, the project aims to understand how genetic variants in humans and patients affect the function of hundreds of cellular transporters."

https://re-solute.eu/resolution

>>14912914

9/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Addgene - London Calling

Partnering with LGC.

https://blog.addgene.org/london-calling-addgenes-new-europe-office-in-london

LGC Standards

"LGC Standards is a division of LGC Group, theUK’s designated National Measurement Institutefor chemical and bioanalytical measurements. We are a leadingglobalmanufacturer and service provider of quality assurance and research tools, supporting your analytical needs in more than120 countries worldwide. We use our expertise to produce to the highest standard, including ranges produced under ISO/IEC 17025 and accredited to ISO 17034. Our wide range of reference materials and proficiency testing schemes is complemented by our experience in outsourcing and customs, enabling us to adapt quickly to provide tailored solutions to meet your analytical needs."

https://www.lgcstandards.com/GB/en/About-LGC-Standards

LGC Standards is Global - as archived 11/1/2021

https://www.lgcstandards.com/

LGC Standards is Global - as archived 8/12/16

https://archive.md/tDxZC

LGC Locations

https://www.lgcgroup.com/about-us/locations/

>>14912914

9/9

Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Zhang Lab

https://zlab.bio/

Federal RePORTER - 32 results for Feng Zhang

https://federalreporter.nih.gov/projects/search/?searchId=4a16fa2d34284312a81b82988fe4b65d&searchMode=Smart&resultType=projects&filters=

Broad Institute

Feng Zhang, Ph.D.

Core Institute Member

https://www.broadinstitute.org/bios/feng-zhang

McGovern Institute

Feng Zhang biography

https://mcgovern.mit.edu/profile/feng-zhang/

WBUR News 4-26-18

"CRISPR Wizard Feng Zhang: The Making Of A Sunny Science Superstar"

https://www.wbur.org/news/2018/04/26/feng-zhang-crispr-profile

>>14912914

Mini Bun forBakerSorry, there are 10 of them, not 9. Can't see screen very well right now.

>>14912914, >>14912923, >>14912929, >>14912935, >>14912941, >>14912945, >>14912952, >>14912960, >>14912963, >>14912965 Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

>>14912971

YW

Good Night, it's tomorrow already. Might as well sleep, can't see the screen.

>>14912994

Let's see what happens. ;-)

>>14912953

great post by Gen. Flynn - vid about role played on Iwo Jima by Navajo code talkers

Notable