Anonymous ID: 22c725 Nov. 9, 2021, 6:08 p.m. No.14963525   🗄️.is 🔗kun

Luciferase

 

It has broad applications across various fields of cell and molecular biology—wherever you want to measure or track the expression of a cloned gene. <<<<<<<HELLO

 

https://bitesizebio.com/10774/the-luciferase-reporter-assay-how-it-works/

 

https://wikileaks.org/berats-box/emailid/23199

 

Role of MKP-1 in skeletal muscle regeneration: Elucidated MKP-1 translational

regulation using ChIP and Luciferase reporter assays.

 

This luciferase reporter assay provides a simple and highly sensitive method to determine the responsiveness of the Tet-system. It is quantitative, reproducible and easy to use in DT40 screens with both transient and stable transfections. The reaction catalyzed by firefly luciferase is the oxidation of luciferin in the presence of coenzyme A with concomitant production of a photon that can be measured by a luminometer as relative light units (RLU) or with a less sensitive scintillation counter.

 

https://pubmed.ncbi.nlm.nih.gov/17623934/

 

When a transient or stable transfection assay is developed for a promoter, a primary objective is to quantify promoter strength. Because transfection efficiency in such assays can be low, promoters are commonly fused to heterologous reporter genes that encode enzymes that can be quantified using highly sensitive assays. The reporter protein's activity or fluorescence within a transfected cell population is approximately proportional to the steady-state mRNA level.

 

 

Luciferase Immunoprecipitation System (LIPS) assay is a rapid, simple, and sensitive test to detect antibody response to respiratory syncytial virus

 

https://www.fda.gov/vaccines-blood-biologics/science-research-biologics/luciferase-immunoprecipitation-system-lips-assay-rapid-simple-and-sensitive-test-detect-antibody