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Moar Digs on Addgene - Think Gain of Function

Anons remember from previous digs thatAddgeneat theBroad Instituteis involved with CRISPR technology and fluoresent protein plasmids. Remember,Eric Lander, thePresident’sScience Advisor andDirector of the White HouseOffice of Science and TechnologyPolicy(OSTP) is a Founding Director of the Broad Insitute. We can now definitively add "Gain of Function" to Addgene's resume. There's a lot to dig on here. Who are the researchers involved? Who do they work for? What other companies or non-profits share this kind of scientific information? Where do they get the cell lines they use in their research?

All PB

>>14875343, >>14875353, >>14875363, >>14875384 Eric Lander, and Much More, Needs Digs

All PB

>>14912914, >>14912923, >>14912929, >>14912935, >>14912941, >>14912945, >>14912952, >>14912960, >>14912963, >>14912965 Eric Lander, Broad Institute, CDC, Addgene, Chan Zuckerberg, Zhang Lab, LGC, Standards, CRISPR and fluorescent protein plasmids

Addgene

"A Better Way toShare Science"

"We distribute 104,724 plasmids on behalf of 4,900 labs from around the world. We also produce 705 ready-to-use viral vectors from our plasmid collection. Find what you need for your next experiment."

https://www.addgene.org/

Now for thenew information. A search on Addgene for "Gain of Function" produces 285 results. Click on 152 Plasmids for a breakdown ofGain of Function Plasmids, researchers involved, related publications and more.

Go to

https://www.addgene.org

search for Gain of Function and then click "152 Plasmids" to find pages of related plasmids.

This is the gain of function information for just onescience "sharing"entity. How many moresharingentities exist? With the volume of information here and the "Grant Support" listed (https://pubmed.ncbi.nlm.nih.gov/27595405/) on some of the PubMed pages, taxpayer funding of gain of function research can not be in question.

>>15003270

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Moar Digs on Addgene - Think Gain of Function

Following is a summary of the Gain of function pages at Addgene: researcher name/names, list of Gain of Function plasmids (by ID#) per researcher and the related publications.

Nancy Craig

Plasmid #17257

"Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon n7"

https://www.addgene.org/browse/article/1911/

Oxford Academics

https://academic.oup.com/genetics/article/145/3/573/6053857

PubMed

https://pubmed.ncbi.nlm.nih.gov/9055068/

Christine Mayr

Plasmids #160697, #160698, #160699, #160700

"Gain of Additional BIRC3 Protein Functions through 3ʹ-UTR-Mediated Protein Complex Formation

https://www.addgene.org/Christine_Mayr/

Molecular Cell

https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30180-7

PubMed

https://pubmed.ncbi.nlm.nih.gov/30948266/

Susan Lindquist

Plasmids #1169, 1168, #1166, #1165, #1164, #1163, #1162, #1161, #1160, #1159, #1158, #1156, #1155, #1154, #1153, #1152, #1151, #1150, #1149, #1148, #1147, #1146

"Dominant B7:C9s in Hsp104p reveal crucial roles for the middle region"

https://www.addgene.org/browse/article/30/

Scientific Society Publisher Alliance

https://www.molbiolcell.org/doi/10.1091/mbc.e02-08-0502

PubMed

https://pubmed.ncbi.nlm.nih.gov/14978213/

David Ackerley

Plasmids #60681, #60682

"A gain-of-function positive-selection expression plasmid that enables high-efficiency cloning."

https://www.addgene.org/browse/article/9167/

Springer

https://link.springer.com/article/10.1007%2Fs10529-014-1673-4

PubMed

https://pubmed.ncbi.nlm.nih.gov/25257589/

Marc Montminy

Plasmids #22969, #22968

"CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis."

https://www.addgene.org/browse/article/25535/

American Society for Microbiology

https://journals.asm.org/doi/10.1128/MCB.20.12.4320-4327.2000

PubMed

https://pubmed.ncbi.nlm.nih.gov/10825195/

>>15003270

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Moar Digs on Addgene - Think Gain of Function

Qi-Jun Chen

Plasmids #91714, #91713, #91709, #91708, #91707

"CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis."

https://www.addgene.org/browse/article/25535/

PubMed

https://pubmed.ncbi.nlm.nih.gov/28303459/

Michael Davidson

Plasmids #89330, #89329, #89328, #89327, #89326, #89325, #89324, #89323, #89322, #89321, #89320, #89319, #89318, #57683

"Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics.

https://www.addgene.org/browse/article/8529/

Journal of Neuroscience

https://www.jneurosci.org/content/34/5/1856

PubMed

https://pubmed.ncbi.nlm.nih.gov/24478365/

Robert Bryson-Richardson

Plasmids #98183, #98182, #98181, #98180

"Zebrafish models of BAG3 myofibrillar myopathy suggest a toxic gain of function leading to BAG3 insufficiency."

https://www.addgene.org/browse/article/28189705/

Springer

https://link.springer.com/article/10.1007%2Fs00401-014-1344-5

PubMed

https://pubmed.ncbi.nlm.nih.gov/25273835/

Peter Bass

Plasmids #89330, #89329, #89328, #89327, #89326, #89325, #89324, #89323, #89322, #89321, #89320, #89319, #89318

"Pathogenic mutation of spastin has gain-of-function effects on microtubule dynamics." https://www.addgene.org/browse/article/8529/

Journal of Neuroscience

https://www.jneurosci.org/content/34/5/1856

PubMed

https://pubmed.ncbi.nlm.nih.gov/24478365/

Tyler Jacks

Plasmids #14854, #14853

"Mutant p53 gain of function in two mouse models of Li-Fraumeni syndrome."

https://www.addgene.org/browse/article/1371/

Cell

https://www.cell.com/cell/fulltext/S0092-8674(04)01046-3

PubMed

https://pubmed.ncbi.nlm.nih.gov/15607980/

Anjana Rao

Plasmids #81025, #81024, #81023, #81023, #81022, #81022, #74263, #74262, #74245, #74244

"Cancer-associated ASXL1 mutations may act as gain-of-function mutations of the ASXL1-BAP1 complex."

https://www.addgene.org/browse/article/17973/

Nature Communications

https://www.nature.com/articles/ncomms8307

PubMed

https://pubmed.ncbi.nlm.nih.gov/26095772/

Jonathan Ewbank

Plasmids #106363, #106362, #106358

"Ewbank lab plasmids"

https://www.addgene.org/browse/article/28192619/

Unpublished

Kenneth Miller

Plasmids #110930, #110929, #110878, #110877, #110876

"Mutations That Rescue the Paralysis of Caenorhabditis elegans ric-8 (Synembryn) Mutants Activate the Gαs Pathway and Define a Third Major Branch of the Synaptic Signaling Network"

https://www.addgene.org/browse/article/28193343/

Oxford Academic

https://academic.oup.com/genetics/article/169/2/631/6060299

PubMed

https://pubmed.ncbi.nlm.nih.gov/15489510/

>>15003270

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Moar Digs on Addgene - Think Gain of Function

Joshua Wythe

Plasmids #156417, #156416, #156415, #156414, #156413, #156412, #156411, #156410, #156409, #156408, #156407, #156406, #156405, #156404, #156403, #156402

"Somatic Gain of KRAS Function in the Endothelium is Sufficient to Cause Vascular Malformations that Require MEK but not PI3K Signaling."

https://www.addgene.org/browse/article/28211645/

AHA Journals

https://www.ahajournals.org/doi/10.1161/CIRCRESAHA.119.316500

PubMed

https://pubmed.ncbi.nlm.nih.gov/32552404/

Eric Procko

Plasmids #107498, #107497, #107496, #107495, #107494, #107493, #107492, 107491, #107485, #107484, #107483, #107478, #107477, #107469, #107467, #107461, #107460

"Erik Procko Lab Plasmids"

https://www.addgene.org/browse/article/28192783/

Unpublished

Colin Taylor

Plasmids #128791, #128788, #128787, #128785, #128781, #128780, #128779, #128778, #128777, #128776, #128772, #128771, #128770, #128769

"A genetically encoded toolkit of functionalized nanobodies against fluorescent proteins for visualizing and manipulating intracellular signalling."

https://www.addgene.org/browse/article/28203714/

BMC Biology

https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-019-0662-4

PubMed

https://pubmed.ncbi.nlm.nih.gov/31122229/

Suzanne Paradis

Plasmid #51595

"A loss-of-function analysis reveals that endogenous Rem2 promotes functional glutamatergic synapse formation and restricts dendritic complexity."

https://www.addgene.org/browse/article/7906/

PLOS ONE

From above sauce, click on "article" link, to see this PLOS ONE publication.

PubMed

https://pubmed.ncbi.nlm.nih.gov/23991227/

Christopher Counter

Plasmids #11136, #11135, #11134, #11133, #11132, #11131

"Loss of hPot1 function leads to telomere instability and a cut-like phenotype." https://www.addgene.org/browse/article/417/

Current Biology

https://www.cell.com/current-biology/fulltext/S0960-9822(04)00987-X

PubMed

https://pubmed.ncbi.nlm.nih.gov/23991227/

Thomas Weimbs

Plasmids #99744, #99743

"Soluble syntaxin 3 functions as a transcriptional regulator."

https://www.addgene.org/browse/article/28193375/

Journal of Biological Chemistry

https://www.jbc.org/article/S0021-9258(20)41846-0/fulltext

PubMed

https://pubmed.ncbi.nlm.nih.gov/29475951/

Carlos Barbas

Plasmids #63900, #63901

"Carlos Barbas"

https://www.addgene.org/Carlos_Barbas/

Unpublished

Dong-Er Zhang

Plasmids #12453, #12452, #12450, #12449

"The interferon-inducible ubiquitin-protein isopeptide ligase (E3) EFP also functions as an ISG15 E3 ligase." https://www.addgene.org/browse/article/885/

Journal of Biological Chemistry

https://www.jbc.org/article/S0021-9258(20)41846-0/fulltext

PubMed

https://pubmed.ncbi.nlm.nih.gov/16352599/

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Moar Digs on Addgene - Think Gain of Function

Roberto Giovannoni

Plasmids #74674, #74672

"Simultaneous Overexpression of Functional Human HO-1, E5NT and ENTPD1 Protects Murine Fibroblasts against TNF-alpha-Induced Injury In Vitro."

https://www.addgene.org/browse/article/18100/

PubMed

https://pubmed.ncbi.nlm.nih.gov/26513260/

Emanuela Felley-bosco and Rolf Stahel

Plasmids #108440, #108439, #108438

"Functional genomic screen in mesothelioma reveals that loss of function of BRCA1-associated-protein-1 induces chemoresistance to ribonucleotide reductase inhibition."

https://www.addgene.org/browse/article/28192941/

American Association for Cancer Research

https://mct.aacrjournals.org/content/19/2/552

PubMed

https://pubmed.ncbi.nlm.nih.gov/31619462/

Victor Anggono and Richard Huganir

Plasmids #72575, #72573, #72522

"PICK1 loss of function occludes homeostatic synaptic scaling."

https://www.addgene.org/browse/article/14564/

The Journal of Neuroscience

https://www.jneurosci.org/content/31/6/2188

PubMed

https://pubmed.ncbi.nlm.nih.gov/21307255/

Stephen Smale

Plasmids #15380, #15379, #15378, #15377

"Selective and antagonistic functions of SWI/SNF and Mi-2beta nucleosome remodeling complexes during an inflammatory response."

https://www.addgene.org/browse/article/1516/

Genes & Development

http://genesdev.cshlp.org/content/20/3/282

PubMed

https://pubmed.ncbi.nlm.nih.gov/16452502/

Sandra Pellegrini

Plasmid #139349

"The Stat3-activating Tyk2 V678F mutant does not up-regulate signaling through the type I interferon receptor but confers ligand hypersensitivity to a homodimeric receptor."

https://www.addgene.org/browse/article/28207661/

Journal of Biological Chemistry

https://www.jbc.org/article/S0021-9258(20)81451-3/fulltext

PubMed

https://pubmed.ncbi.nlm.nih.gov/18456658/

GuangJun Zhang

Plasmids #164966, #164965, 164950, #164949, #164947, #164946, #164945, #164944, #164943, #164942, #164941, #164940

"Potassium Channel-Associated Bioelectricity of the Dermomyotome Determines Fin Patterning in Zebrafish." https://www.addgene.org/browse/article/28215839/

Oxford Academy

https://academic.oup.com/genetics/article/215/4/1067/6065547

PubMed

https://pubmed.ncbi.nlm.nih.gov/32546498/

Joan Conaway

Plasmids #173945, #173944, #173943, #173942, #173941, #173940, #173939, #173938, #173937, #173936, #173935, #173934, #173933, #173932

"Multiple roles for PARP1 in ALC1-dependent nucleosome remodeling."

https://www.addgene.org/browse/article/28220027/

Proceedings of the National Academy of Sciences of the United States of America https://www.pnas.org/content/118/36/e2107277118

PubMed

https://pubmed.ncbi.nlm.nih.gov/34465625/

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Moar Digs on Addgene - Think Gain of Function

Bob Weinberg

Plasmids #19127, #19126, #19125, #19123, #19122, #19120, #19119

"Growth-inhibitory and tumor- suppressive functions of p53 depend on its repression of CD44 expression." https://www.addgene.org/browse/article/2244/

Cell

https://www.cell.com/cell/fulltext/S0092-8674(08)00754-X

PubMed

https://pubmed.ncbi.nlm.nih.gov/18614011/

Ken Haynes

Plasmids #61768, #61767, #61766, #61765

"Exploitation of sulfonylurea resistance marker and non-homologous end joining mutants for functional analysis in Zymoseptoria tritici."

https://www.addgene.org/browse/article/14777/

Science Direct

https://www.sciencedirect.com/science/article/pii/S108718451500081X

PubMed

https://pubmed.ncbi.nlm.nih.gov/26092796/

Yubin Zhou

Plasmids #69552, #69551, #68403

"Inside-out Ca(2+) signalling prompted by STIM1 conformational switch."

https://www.addgene.org/browse/article/14799/

Nature Communications

https://www.nature.com/articles/ncomms8826

PubMed

https://pubmed.ncbi.nlm.nih.gov/26184105/

Rene Verwaal

Plasmids #101750, #101749, #101748, # 101725

"CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae." https://www.addgene.org/browse/article/28192247/

Wiley Online Library

https://onlinelibrary.wiley.com/doi/10.1002/yea.3278

PubMed

https://pubmed.ncbi.nlm.nih.gov/28886218/

George Church

Plasmids #80944, #80943, #80942, #80941, #80940, #80939, #80938, #80937, #80936, #80935, #80934, #80933, #80932, #80931, #80930

"A multifunctional AAV-CRISPR-Cas9 and its host response."

https://www.addgene.org/browse/article/22281/

Nature Methods

https://www.nature.com/articles/nmeth.3993

PubMed

https://pubmed.ncbi.nlm.nih.gov/27595405/

Maria Alvarado-Kristensson

Plasmid #66886

"Tumors with nonfunctional retinoblastoma protein are killed by reduced gamma-tubulin levels." https://www.addgene.org/browse/article/11590/

Journal of Biological Chemistry

https://www.jbc.org/article/S0021-9258(20)50246-9/fulltext

PubMed

https://pubmed.ncbi.nlm.nih.gov/22493456/

>>15003270

Baker

Here's all 6 of them,

for your convenience.

>>15003270, >>15003281, >>15003295, >>15003304, >>15003312, >>15003320 Moar Digs on Addgene - Think Gain of Function

>>15003305

>>15003311 >nice work, science-fag!

>>15003352

TY Frens

Plain old Anon here digging in the rabbit holes.