Anonymous ID: c415d3 Feb. 13, 2022, 8:28 p.m. No.15622708   🗄️.is 🔗kun   >>2717 >>2721 >>2730 >>2752 >>2841 >>2888 >>3062 >>3221 >>3268

https://www.sciencedirect.com/science/article/pii/S2001037022000319

Computational and Structural Biotechnology Journal

Volume 20, 2022, Pages 799-811

Computational and Structural Biotechnology Journal

Montelukast is a dual-purpose inhibitor of SARS-CoV-2 infection and virus-induced IL-6 expression identified by structure-based drug repurposing

 

Abstract

Drug-repurposing has been instrumental to identify drugs preventing SARS-CoV-2 replication or attenuating the disease course of COVID-19. Here, we identify through structure-based drug-repurposing a dual-purpose inhibitor of SARS-CoV-2 infection and of IL-6 production by immune cells. We created a computational structure model of the receptor binding domain (RBD) of the SARS-CoV-2 spike 1 protein, and used this model for insilico screening against a library of 6171 molecularly defined binding-sites from drug molecules. Molecular dynamics simulation of candidate molecules with high RBD binding-scores in docking analysis predicted montelukast, an antagonist of the cysteinyl-leukotriene-receptor, to disturb the RBD structure, and infection experiments demonstrated inhibition of SARS-CoV-2 infection, although montelukast binding was outside the ACE2-binding site. Molecular dynamics simulation of SARS-CoV-2 variant RBDs correctly predicted interference of montelukast with infection by the beta but not the more infectious alpha variant. With distinct binding sites for RBD and the leukotriene receptor, montelukast also prevented SARS-CoV-2-induced IL-6 release from immune cells. The inhibition of SARS-CoV-2 infection through a molecule binding distal to the ACE-binding site of the RBD points towards an allosteric mechanism that is not conserved in the more infectious alpha and delta SARS-CoV-2 variants.

 

Authors

MaxLuedemanna1DanielaStadlerb1Cho-ChinChengbUlrikeProtzerbcdPercy A.KnolleadSainitinDonakondaad2

Anonymous ID: c415d3 Feb. 13, 2022, 8:32 p.m. No.15622730   🗄️.is 🔗kun   >>2841 >>2888 >>2934 >>3062 >>3221 >>3268

>>15622708

>>15622717

https://www.medscape.com/viewarticle/967634

 

Postvaccination Multisystem Inflammatory Syndrome in Adult With No Evidence of Prior SARS-CoV-2 Infection

Young Kyun Choi; Jae Young Moon; Jungok Kim; In Seol Yoo; Geun-Yong Kwon; Heuisoon Bae; Min Seob Song; Sungmin Kym

 

DISCLOSURES Emerging Infectious Diseases. 2022;28(2):411-414.

 

Abstract

Ten days after receiving the first dose of coronavirus disease vaccine, a 22-year-old woman in South Korea experienced myocarditis, myopathy, pericarditis, and gastroenteritis; rash subsequently developed. There was no evidence of prior infection with severe acute respiratory syndrome coronavirus 2. The diagnosis was multisystem inflammatory syndrome resulting from coronavirus disease vaccination.

Anonymous ID: c415d3 Feb. 13, 2022, 8:35 p.m. No.15622742   🗄️.is 🔗kun   >>2753 >>2757 >>2792 >>2841 >>2888 >>3062 >>3221 >>3268

MIT Technology Review has a new piece by Jane Qiu on Shi Zhengli, the renowned Wuhan virologist at the centre of the lab leak. Qiu is Shi’s personal propagandist, responsible for the notorious 2020 Scientific American piece on How China’s ‘Bat Woman’ Hunted Down Viruses From SARS to the New Coronavirus.

 

In her latest whitewash, Qiu explains that Shi has granted her “unparalleled” access, because of her “strong science background” which “allows [her] to grasp the nuances and complexity” of what it is that Shi and her den of virologists get up to. Qiu further admits that her purpose is not to investigate anything; instead, she wants “Shi and her team [to] tell their side of the story on the record, and in the most detail to date.” As you might expect, nobody at the Wuhan Institute of Virology has any interest in disclosing new information, which leaves Qiu with little to report that we didn’t know already. Just a lot of the same old dissimulations, with the occasional update.

 

The most interesting of these surrounds the Mystery of the Mojiang Cave. The story goes that workers were cleaning up bat droppings in this abandoned copper mine in Yunnan province in the spring of 2012, when six of them fell ill with pneumonia. Shi’s lab tested blood samples to see if they’d contracted SARS or a SARS-related virus, and afterwards the Wuhan virologists developed a persistent interest in the Mojiang mine. Among the bat samples collected there, they claim to have found RaTG13, the closest known relative of SARS-2.

 

Note all of the bizarre coincidences you must live with, if you believe SARS-2 has natural origins: You have to imagine the virus just happened to enter humans via some zoonotic event in Wuhan, the only city on earth with a lab devoted to sampling and culturing SARS-related bat coronaviruses like SARS-2, where its closest known relative also just happened to be sitting in a freezer. And we haven’t even gotten to the furin cleavage site yet.

 

It is curious, then, that nobody can ever get the story straight, about what happened with those mine workers. In 2020, Shi’s line was that the miners had a fungal infection of some kind, and never tested positive for SARS antibodies. Then The Seeker, an anonymous Twitter investigator, uncovered this pre-pandemic Ph.D. thesis from 2016, which states plainly that the Wuhan Institute of Virology indeed detected SARS antibodies in their blood. Thereafter George Gao, head of the Chinese CDC, conceded to French reporters that the miners had antibodies to a SARS-related virus, but suggested (implausibly) that these might have been caused by some prior infection and they might well have nothing to do with the cave.

 

In Qiu’s article we find the latest excuse: “Shi said her team did not find such antibodies, although she said some early tests did produce false positives that were corrected when the assays were fully validated.” So, the miners tested positive for SARS, before they tested negative for SARS. The only problem with this lie, is that it can’t explain why Shi and her team ever took an interest in the Mojiang mine in the first place:

 

It’s not unusual for respiratory illnesses to have an unknown cause, but even though Shi couldn’t figure out what had sickened the Mojiang miners, her instinct told her that something interesting might be going on. “What viruses were lurking in the cave?” she remembers wondering. Between 2012 and 2015, her team undertook more than half a dozen trips to the mine shaft, about 1,100 miles from Wuhan, and collected 1,322 bat samples.

 

Emphasis mine. Shi found nothing in the miners’ blood, but decided nevertheless that the distant cave where they got sick was the perfect place to spend three years sampling bats for SARS-related coronaviruses. Totally by coincidence, bats in the cave turned out to be full of SARS-related coronaviruses, including RaTG13.

 

The virologists are not troubled by any of that, of course. Economies and lives may lie in ruins, but it is the personal plight of Shi Zhengli, the Bat Lady from Wuhan, that worries them above all. We need more cooperation. We need less prejudice. That’s what The Science says.

 

https://www.eugyppius.com/p/the-bat-lady-from-wuhan-and-the-very

Anonymous ID: c415d3 Feb. 13, 2022, 9:09 p.m. No.15622911   🗄️.is 🔗kun   >>2926 >>2932 >>2939 >>3001

>>15622812

she spicy

https://www.newsweek.com/actress-rosario-dawson-accused-holding-down-trans-employee-during-beating-misgendering-him-1466796

 

Actress Rosario Dawson Accused of Holding Down Trans Employee During Beating, Misgendering Him

BY MELISSA LEMIEUX ON 10/21/19 AT 6:26 PM EDT

 

Actress Rosario Dawson is being sued by an ex-friend and employee, who has accused her of beating and misgendering him.

 

Dedrek Finey, who knew Rosario Dawson's family for years and helped renovate the actress' home after her mother encouraged him to move cross-country from California to New York to work for them, has accused Dawson of attacking, harassing, misgendering, beating and punching him.

Finey opened up about his transition to Dawson and her family after he moved to Los Angeles, at which point Finey was repeatedly allegedly misgendered by members of the Dawson family.

 

Dawson's mother, Isabel, her father, Gregory and her brother Gustavo all refused to use he/him pronouns around Finey and deadnamed him, even though Finey repeatedly corrected them.

 

When Finey complained to Dawson about the misgendering, she, too allegedly repeatedly misgendered him. When he protested, she replied, "You're a grown woman." When Finey tried to correct her pronoun usage, Dawson allegedly replied "Whatever."

After having his hours reduced and being told to move out of a house on Dawson's property in 2018, where he was living rent-free in exchange for doing the home renovations, he alleges the actress' mother, Isabelle, assaulted him on April 28 when he refused to discuss the matter with her.

The suit alleges that Isabel threatened to kill his cat, then ripped a window screen out and pulled Finey through the window. She then allegedly began punching Finey, saying "You're not so much of a man now."

 

At that point Dawson told her mother, "Mom, stop being petty." According to Finey, Dawson then pinned him down so her mother could continue to beat him.

 

Finey then claims that one of the women took his phone away during the beating, because they knew Finey had a video of Isabel threatening Finey from earlier in the year and to keep him from dialing 911.

 

Finey eventually escaped, at what point he rushed into Dawson's house and called the police. The police transported Finey to the hospital, and he subsequently received a restraining order against Isabel. He accuses the family of shutting his gas off in September, forcing him to move out of the house and making his living conditions within the guest house uninhabitable.

 

Finey is suing Rosario Dawson, Isabel Dawson and Dawson's uncle and stepfather for assault, battery, trespass, discrimination, civil rights and labor violations.