Experimental methods[edit]
The high degree of homology between D5 and D1 receptors and their affinity for drugs with similar pharmacological profile complicate distinguishing between them in research. Antibody staining these two receptors separately is suggested to be inefficient.[44] However, expression of D5 receptors has been assessed using immunohistochemistry. In this technique, two peptides were obtained from third exracellular loop and third intracellular loop of the receptor, and antisera were developed for staining the receptor in frozen mouse brain tissue.[35] A method involving mRNA probes for in situ hybridization has been developed, which allowed to separately examine the expression of D1 and D5 receptors in the mouse brain.[24]
DRD5 knockout mice can be obtained by crossing 129/SvJ1 and C57BL/6J mice.[10] D5 receptor can also be inactivated in an animal model by flanking the DRD5 gene with loxP site, allowing to generate tissue or animal lacking functional D5 receptors.[45] The expression of D5 receptor in vitro can also be silenced using antisense oligonucleotides.[20]
Dopamine receptor D5, also known as D1BR, is a protein that in humans is encoded by the DRD5 gene.[5] It belongs to the D1-like receptor family along with the D1 receptor subtype.
https://en.wikipedia.org/wiki/Dopamine_receptor_D5#Inverse_agonists