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Abstract
Lactococcosis is a disease encountered in a wide variety of fish species causing mortalities and having great economic impact on farmed fish. In this study, we report for the first time the isolation of a strain of the recently described novel species Lactococcus petauri, from rainbow trout suffering from lactococcosis. The aim of this study was to determine the complete genome sequence of L. petauri strain LG_SAV_20 and to characterize its antimicrobial resistance and virulence. The genome of L. petauri LG_SAV_20 consists of 2,078,949 base pair (bp) with a GC content of 38.05%, 1950 predicted coding sequence (CDS), and 60 RNAs (51 tRNAs, 3 ncRNAs, and 6 rRNAs). Phylogenetic analysis revealed that L. petauri LG_SAV_20 shares most of its genome with L. garvieae strains isolated from rainbow trout. Detection of genes associated with antimicrobial resistance indicated that the isolate possesses the multidrug transporter mdt(A) gene, while using comparative analysis we identified several genes that might be related to bacterial pathogenesis. This genomic information provides new insights into the role of this novel species as an etiological agent of lactococcosis.
https://pubmed.ncbi.nlm.nih.gov/32008079/
Zelensky looks like a Prisoner of War!
1 Jun, 2022 12:52
Kremlin explains why it doesn't trust Zelensky
President Zelensky cannot be trusted not to use weapons to attack targets in Russia, Moscow says
Ukraine cannot be trusted to abide by Washington’s wishes and not use US-supplied rocket launchers against targets in Russia, Kremlin spokesman Dmitry Peskov said on Wednesday. Kiev has a poor record on keeping its word, the official claimed.
Peskov was commenting on the US decision to provide Ukraine with HIMRAS multiple rocket launchers. US officials stated that the weapon systems would not be used against targets in Russia, arguing that this made Washington not a party to the Ukraine conflict.
Ukraine’s President Volodymyr Zelensky pledged to honor the US assurances when he spoke to Newsmax.
“We’re not interested in what’s happening in Russia,” he promised in an interview published on Wednesday. “We’re only interested in our own territory in Ukraine.”
When asked about the promise, Peskov said Russia didn’t trust Zelensky to keep his word.
“Unfortunately, such a thing is not what our experience suggests,” he said. Zelensky has been breaking his word throughout his relatively short political career, “starting with his main election campaign promise to put an end to the war in southeastern Ukraine,” Peskov said.
Zelensky, a former comedian, made a surprise switch to politics in January 2019, announcing his decision to run for president. He quickly gained momentum in the race, partly because he had played a character in a political comedy show, who took power in Ukraine by a chance and used it to take down entrenched corruption in the government while challenging global institutions like the International Monetary Fund.
The unorthodox candidate pledged to reconcile with rebel forces of the Donbass, who turned against the central government after the 2014 armed coup in Kiev. He received an unprecedented level of public support, scoring over 73% of votes in the second round face-off against incumbent President Petro Poroshenko.
Instead of delivering peace, Zelensky continued his predecessor’s policies regarding the breakaway republics and openly refused to implement the Minsk agreements, an EU-brokered peace roadmap for the Donbass.
Considering the circumstances “we give no credit of trust to the Ukrainian side,” Peskov said. Russia does not believe that the US was acting in good faith either, the Kremlin spokesman added.
“The US is pouring gasoline on the fire deliberately and with enthusiasm,” and American arms supplies “encourage the Ukrainian leadership not to resume peace talks,” he said.
Peskov pledged that the Russian military will take precautions to minimize the potential threat posed to Russia by American missiles in Ukraine’s possession.
https://www.rt.com/russia/556436-zelensky-trust-weapons-russia/