Ionizable lipid nanoparticles for in utero mRNA delivery

https://www.lens.org/lens/scholar/article/003-007-100-218-842/main

Abstract

Clinical advances enable the prenatal diagnosis of genetic diseases that are candidates for gene and enzyme therapies such as messenger RNA (mRNA)–mediated protein replacement. Prenatal mRNA therapies can treat disease before the onset of irreversible pathology with high therapeutic efficacy and safety due to the small fetal size, immature immune system, and abundance of progenitor cells. However, the development of nonviral platforms for prenatal delivery is nascent. We developed a library of ionizable lipid nanoparticles (LNPs) for in utero mRNA delivery to mouse fetuses. We screened LNPs for luciferase mRNA delivery and identified formulations that accumulate within fetal livers, lungs, and intestines with higher efficiency and safety compared to benchmark delivery systems, DLin-MC3-DMA and jetPEI. We demonstrate that LNPs can deliver mRNAs to induce hepatic production of therapeutic secreted proteins. These LNPs may provide a platform for in utero mRNA delivery for protein replacement and gene editing.

https://mobile.twitter.com/fynn_fan/status/1603491883413561361

Dr. Lynn Fynn (Fan Account)🐭

@fynn_fan

Buckle up parents! Better stand up!

https://mobile.twitter.com/JustinTrudeau/status/1603504463049801728

Justin Trudeau

@JustinTrudeau

Officiel du gouvernement - Canada

If you’ve got a question for me, now’s the time to ask it. Send it in by replying to this post – and then stay tuned!

https://twitter.com/ElectionWiz/status/1603556529499783170

Election Wizard 🇺🇸

@ElectionWiz

REPORT: Twitter has suspended the account of prominent liberal journalist Aaron Rupar as well as reporters from CNN, The New York Times and The Washington Post.

>>17953528

https://twitter.com/robkroese/status/1603584998510051329

Robert Kroese

@robkroese

Lot of journalists out here worrying about being suspended who didn't say a damn thing when the New York Post was suspended for publishing a story about Hunter Biden's laptop. Weird

>>17953396

probably why congress need not declare war as there is an ongoing war on terror. Just label a target a terrorist state, or state sponsor of terror, and get busy with funding and warring- no further approvals or votes needed.

>>17953513

Jan 5 New York State Legislative Session Docket

>>17953561

yes, Allah means God in Arabic.

>>17953601

God is truth yes.

Allah is an Arabic word for God.

Is this difficult?

Allah is the common Arabic word for God. In the English language, the word generally refers to God in Islam. The word is thought to be derived by contraction from al-ilāh, which means "the god", and is linguistically related to the Aramaic words Elah and Syriac ܐܲܠܵܗܵܐ and the Hebrew word El for God.

>>17953614

yes, God is the truth. This is new/shocking to you?

Allah is the unique, omnipotent and only deity and creator of the universe and is equivalent to God in other Abrahamic religions.

God

Allah

Hashem

etc

different languages same creator

>>17953624

talked about being able to predict now in last night's q & a

>>17953641

the words god/allah are defining the same creator origin of all.

what words man attributes to them to domesticate, colonize, and rule, are tools and works of men, for purposes of domestication and control.

>>17953633

stupid post?

backpedal?

sauce?

Praise God indeed! There is nothing else.

u seem mad, y tho?

>>17953676

men wrote everything you stated. Imperfect, 'sinners'.

the word for the creator exists in many languages. Islam and Christianity are not God, you understand this, yes?

>>17953711

https://petermcculloughmd.substack.com/p/postural-orthostatic-tachycardia

Postural Orthostatic Tachycardia Syndrome (POTS)

Trouble with Heart Rate and Blood Pressure

after COVID-19, Vaccination, or Both

One of the most common symptomatic complaints after COVID-19, vaccination, and now in most persons who have both exposures is POTS (postural orthostatic tachycardia syndrome). This is a disorder where there is a lack of synchronization between the heart, brain, spinal cord sympathetic chain of ganglia, adrenal gland, and the blood vessels both arterial and venous. The Spike protein on SARS-CoV-2 and flooding the system after vaccination damages neurological tissue, the adrenal glands, and the heart. The end result can be inappropriately elevated heart rate and either low or high blood pressure causing dizziness. I have seen cases of syncope with facial trauma as a result of this bothersome condition. Kwan et al has shown (n=284,592) that the risk for POTS is substantial, just after heart damage as the highest risk cardiovascular side effect of COVID-19 vaccination.

Most of the care for POTS is supportive (lower leg compression stockings, increased salt and fluid intake, exercise). I have found in severe cases with adequate blood pressure that the use of nadolol can control the tachycardia and help while the patient has cardiovascular-neurological reflexes return to normal over a period of months. Since most children and adults in the US have recovered from COVID-19, there is no reason to take additional vaccines and risk POTS, myocarditis, or both.

If you find “Courageous Discourse” enjoyable and useful to your endeavors, please subscribe as a paying or founder member to support our efforts in helping you engage in these discussions with family, friends, and your extended circles.

Kwan, A.C., Ebinger, J.E., Wei, J. et al. Apparent risks of postural orthostatic tachycardia syndrome diagnoses after COVID-19 vaccination and SARS-Cov-2 Infection. Nat Cardiovasc Res (2022). https://doi.org/10.1038/s44161-022-00177-8

Courageous Discourse™ with Dr. Peter McCullough & John Leake is a reader-supported publication. To receive new posts and support my work, consider becoming a free or paid subscriber.

Ivermectin converts cold tumors hot and synergizes with immune checkpoint blockade for treatment of breast cancer

https://www.nature.com/articles/s41523-021-00229-5

Abstract

We show that treatment with the FDA-approved anti-parasitic drug ivermectin induces immunogenic cancer cell death (ICD) and robust T cell infiltration into breast tumors. As an allosteric modulator of the ATP/P2X4/P2X7 axis which operates in both cancer and immune cells, ivermectin also selectively targets immunosuppressive populations including myeloid cells and Tregs, resulting in enhanced Teff/Tregs ratio. While neither agent alone showed efficacy in vivo, combination therapy with ivermectin and checkpoint inhibitor anti-PD1 antibody achieved synergy in limiting tumor growth (p = 0.03) and promoted complete responses (p < 0.01), also leading to immunity against contralateral re-challenge with demonstrated anti-tumor immune responses. Going beyond primary tumors, this combination achieved significant reduction in relapse after neoadjuvant (p = 0.03) and adjuvant treatment (p < 0.001), and potential cures in metastatic disease (p < 0.001). Statistical modeling confirmed bona fide synergistic activity in both the adjuvant (p = 0.007) and metastatic settings (p < 0.001). Ivermectin has dual immunomodulatory and ICD-inducing effects in breast cancer, converting cold tumors hot, thus represents a rational mechanistic partner with checkpoint blockade.

>>17953737

>>17953746

perhaps a T-cell-specific targeting parasite is the culprit. Parasitic amoeba perhaps…

off to dig

>>17953778

>>17953746

>>17953765

>>17953737

GenusTrypanosomafrom classKinetoplastidacan directly affect the human immune system, and specifically, T cell function.

https://www.icliniq.com/articles/immune-disorders/t-cell-disorders

Trypanosoma is a genus of kinetoplastids (class Trypanosomatidae[1]), a monophyletic[2] group of unicellular parasitic flagellate protozoa. Trypanosoma is part of the phylum Sarcomastigophora.[3] The name is derived from the Greek trypano- (borer) and soma (body) because of their corkscrew-like motion. Most trypanosomes are heteroxenous (requiring more than one obligatory host to complete life cycle) and most are transmitted via a vector (mosquito, tick, etc). The majority of species are transmitted by blood-feeding invertebrates, but there are different mechanisms among the varying species. Some, such as Trypanosoma equiperdum, are spread by direct contact. In an invertebrate host they are generally found in the intestine, but normally occupy the bloodstream or an intracellular environment in the vertebrate host.

https://en.wikipedia.org/wiki/Trypanosoma

Kinetoplastida (or Kinetoplastea, as a class) is a group of flagellated protists belonging to the phylum Euglenozoa,[3][4] and characterised by the presence of an organelle with a large massed DNA called kinetoplast (hence the name). The organisms are commonly referred to as "kinetoplastids" or "kinetoplasts"[5] The group includes a number of parasites responsible for serious diseases in humans and other animals, as well as various forms found in soil and aquatic environments. Their distinguishing feature, the presence of a kinetoplast, is an unusual DNA-containing granule located within the single mitochondrion associated with the base of the cell's flagellum (the basal body). The kinetoplast contains many copies of the mitochondrial genome.

https://en.wikipedia.org/wiki/Kinetoplastida

PARASITES

>>17953961

>when someone shows you who they are, believe them the first time.

“Over time I think we will probably see a closer merger of biological intelligence and digital intelligence,” Musk told an audience at the World Government Summit in Dubai, where he also launched Tesla in the United Arab Emirates (UAE).

https://www.cnbc.com/2017/02/13/elon-musk-humans-merge-machines-cyborg-artificial-intelligence-robots.html

https://www.teslarati.com/elon-musk-shares-tesla-mrna-microfactories-details/#google_vignette

https://twitter.com/elonmusk/status/1325934016353214465

>>17954047

>They

you are not anon? then identify pls.