https://www.biorxiv.org/content/10.1101/2022.12.01.518149v2.full

SARS-CoV-2 mRNA vaccine is re-adenylated in vivo, enhancing antigen production and immune response

Abstract

Though mRNA vaccines against COVID-19 have revolutionized vaccinology and have been administered in billions of doses, we know incredibly little about how mRNA vaccines are metabolized in vivo. Here we implemented enhanced nanopore Direct RNA sequencing (eDRS), to enable the analysis of single Moderna’s mRNA-1273 molecules, giving in vivo information about the sequence and poly(A) tails.

We show that mRNA-1273, with all uridines replaced by N1-methylpseudouridine (mΨ), is terminated by a long poly(A) tail (~100 nucleotides) followed by an mΨCmΨAG sequence. In model cell lines, mRNA-1273 is swiftly degraded in a process initiated by the removal of mΨCmΨAG, followed by CCR4-NOT-mediated deadenylation. In contrast, intramuscularly inoculated mRNA-1273 undergoes more complex modifications. Notably, mRNA-1273 molecules are re-adenylated after mΨCmΨAG removal. Detailed analysis of immune cells involved in antigen production revealed that in macrophages, after mΨCmΨAG removal, vaccine mRNA is very efficiently re-adenylated, and poly(A) tails can reach up to 200A. In contrast, in dendritic cells, vaccine mRNA undergoes slow deadenylation-dependent decay. We further demonstrate that enhancement of mRNA stability in macrophages is mediated by TENT5 poly(A) polymerases, whose expression is induced by the vaccine itself. Lack of TENT5-mediated re-adenylation results in lower antigen production and severely compromises specific immunoglobulin production following vaccination.

Together, our findings provide an unexpected principle for the high efficacy of mRNA vaccines and open new possibilities for their improvement. They also emphasize that, in addition to targeting a protein of interest, the design of mRNA therapeutics should be customized to its cellular destination.

https://www.biorxiv.org/content/10.1101/2022.12.01.518149v2.full.pdf

https://summit.news/2023/02/06/surfing-icon-says-shell-boycott-professional-tour-over-transgender-competitors

Surfing Icon Says She’ll Boycott Professional Tour Over Transgender Competitors

Says hormone levels alone don’t determine what a woman is.

American surfing icon Bethany Hamilton has vowed to boycott the professional tour over a rule change that allows biological males who identify as women to compete against female surfers.

Hamilton took the stance after the World Surf League (WSL) announced it would comply with the policies of the International Surfing Association (ISA) when it comes to transgender surfers.

Athletes born men can compete in female divisions if they maintain a testosterone level less than five nmol/L (nanomoles per litre) for 12 months.

WSL chief Jessi Miley-Dyer said the policy was important in order to “balance equity and fairness.”

However, Hamilton took to Instagram to complain that testosterone levels alone don’t determined what a woman is.

“Is a hormone level an honest and accurate depiction that someone indeed is a male or female? Is it as simple as this?” she asked.

Surfing champion Bethany Hamilton has just announced she will NOT compete in the World Surf League anymore if they allow biological men to surf in the women’s events. She’s already an absolute legend but this stand makes her even more of a legend. Thank you @bethanyhamilton! pic.twitter.com/gF2qa7cD84

— Robby Starbuck (@robbystarbuck) February 5, 2023

Hamilton speaking out on the issue is a big deal because of her high profile and success in the sport. She has been featured in a number of television specials and documentaries after losing her arm to a shark attack when she was a child and overcoming it to compete at the highest level.

“I personally won’t be competing in or supporting the World Surf League if this rule remains,” said Hamilton, adding, “This concerns me as a professional athlete who has been competing for the last 15-plus years.”

The surfer said she decided to go public in order to “stand up and speak up for those in a position that may feel they can’t say something about this.”

“I think many of the girls who are on tour are not in support of this new rule, and they fear being ostracised if they speak up,” said Hamilton.

She also noted how sports where similar policies were introduced, such as swimming, running, and MMA, were being dominated by biological men identifying as transgender.

“Who is pushing for this huge change? Does this better the sport of surfing? Is this better for the women in surfing? If so, how?” she demanded to know.

Hamilton argued that a new division should be created solely for transgender athletes in order to protect the female division.

“I think it’s really hard to imagine what the future of women’s surfing will be like in 15-20 years down the road if we move forward allowing this major change,” she concluded.

As we highlighted last year, swimming’s world governing body slapped a total ban on transgender athletes that have gone through any form of male puberty from taking part in women’s competitions.

https://mobile.twitter.com/franklin_reeder/status/1622626689720123396

Mark Reeder

@franklin_reeder

·

Feb 6

Replying to

@andrewbostom

@anish_koka

and

@TracyBethHoeg

How many deaths were among vaxed who later tested + ?

They were counted as SARS-caused.

@Jikkyleaks

“If an individual had received an mRNA vaccine and had a positive PCR test result for SARS- CoV-2 infection within 28 days, the latest exposure defined the type of myocarditis.”

Mark Reeder

@franklin_reeder

Replying to

@franklin_reeder

@andrewbostom

and 3 others

How many people died after:

(1) getting MRNA vax, then

(2) getting Covid, then

(3) getting myocarditis within 28 days of the vaccine?

Yet their myocarditis was classified as “due to Covid” in this study!

An honest person would say the vaccine is the cause! Enough of the lies!😡

https://mobile.twitter.com/StarkNakedBrief/status/1622561134824882176

The Stark Naked Brief.

@StarkNakedBrief

[ REPORT ] - New evidence has emerged suggesting BioNTech and Pfizer falsified key data in their submissions to the European Medicines Agency and the Federal Drugs Agency, which allowed them to secure emergency use authorisation…

The Stark Naked Brief.

@StarkNakedBrief

·

Feb 6

Replying to

@StarkNakedBrief

The key data in question regards what is known as "Western blot" analysis

A western blot is a technique used to identify and separate specific proteins from a mixture of proteins extracted from cells, based on their molecular weights.

The Stark Naked Brief.

@StarkNakedBrief

·

Feb 6

If true, this could break Pfizer's indemnity protection from legal liability resulting from deaths or injuries caused by their product

Breakdown via

@sonia_elijah

trialsitenews.com

Startling Evidence Suggests BioNTech and Pfizer Falsified Key Data: Part 1

Evidence has emerged casting serious doubt over the authenticity of tests carried out by BioNTech (Marketing Authorisation Holder) and Pfizer to prove the fidelity of their product by demonstrating…

>>18300769

Clinical outcomes of myocarditis after SARS-CoV-2 mRNA vaccination in four Nordic countries: population based cohort study

https://bmjmedicine.bmj.com/content/2/1/e000373.abstract?ct=

Abstract

Objective To investigate the clinical outcomes of myocarditis associated with mRNA vaccines against the SARS-CoV-2 virus compared with other types of myocarditis.

Results In 2018-22, 7292 patients were admitted to hospital with new onset myocarditis, with 530 (7.3%) categorised as having myocarditis associated with SARS-CoV-2 mRNA vaccination, 109 (1.5%) with myocarditis associated with covid-19 disease, and 6653 (91.2%) with conventional myocarditis. At the 90 day follow-up, 62, nine, and 988 patients had been readmitted to hospital in each group (vaccination, covid-19, and conventional myocarditis groups, respectively), corresponding to a relative risk of readmission of 0.79 (95% confidence interval 0.62 to 1.00) and 0.55 (0.30 to 1.04) for the vaccination type and covid-19 type myocarditis groups, respectively, compared with the conventional myocarditis group. At the 90 day follow-up, 27, 18, and 616 patients had a diagnosis of heart failure or died in the vaccination type, covid-19 type, and conventional myocarditis groups, respectively. The relative risk of heart failure within 90 days was 0.56 (95% confidence interval 0.37 to 0.85) and 1.48 (0.86 to 2.54) for myocarditis associated with vaccination and covid-19 disease, respectively, compared with conventional myocarditis; the relative risk of death was 0.48 (0.21 to 1.09) and 2.35 (1.06 to 5.19), respectively. Among patients aged 12-39 years with no predisposing comorbidities, the relative risk of heart failure or death was markedly higher for myocarditis associated with covid-19 disease than for myocarditis associated with vaccination (relative risk 5.78, 1.84 to 18.20).

Conclusions Compared with myocarditis associated with covid-19 disease and conventional myocarditis, myocarditis after vaccination with SARS-CoV-2 mRNA vaccines was associated with better clinical outcomes within 90 days of admission to hospital.

AZ 🛰🌏🌍🌎

@AZgeopolitics

🇺🇸🇷🇺🇨🇦🇬🇧Yevgeniy Prigozhin latest press release he is considering USA,UK and Canada goverment bodies illegitimate

Promises "Wagner" to help all the opressed people of these countries

https://twitter.com/AZgeopolitics/status/1622246717184688130

https://twitter.com/jenniferzeng97/status/1622935718199689216

https://twitter.com/jenniferzeng97/status/1622932279998685185

https://twitter.com/jenniferzeng97/status/1622932281106042880

Inconvenient Truths by Jennifer Zeng 曾錚真言

@jenniferzeng97

Females need to serve too, except during pregnancy, maternity leave, and breastfeeding.

The shortest serving time is 4 years.

Ordinary reservists can apply for discharge after reaching the age of 30. Officers need to serve until 45-60, depending on their ranks.

Quote Tweet

Inconvenient Truths by Jennifer Zeng 曾錚真言

@jenniferzeng97

·

5h

1. Ready for #war?

#CCP has quietly passed its Reserve Military Service Law, which will take effect on 3/1/2023.

Anyone who is 18 years or older is obliged to enlist after a national defense mobilization is announced. "After receiving the call-up notice, reservists must report to

Show this thread

Inconvenient Truths by Jennifer Zeng 曾錚真言

@jenniferzeng97

1. the designated place at the specified time in accordance with the requirements. After the state issued the mobilization order, the reserve personnel who have not received the call-up notice shall not leave the reserve registration place without the approval of

Inconvenient Truths by Jennifer Zeng 曾錚真言

@jenniferzeng97

1. the troops and the military service organs of the reserve registration place; if they have left, they shall return immediately or standby in the same place."

Full text: http://npc.gov.cn/npc/c30834/202212/675bfdf572d1440d89e29080e7310b6f.shtml

Now you understand more about the #ChineseSpyBalloon?

https://twitter.com/VDAREJamesK/status/1620236088500682752

James Kirkpatrick

@VDAREJamesK

This is important. If they were "traitors," you would think the federal government would put them on trial. They didn't because secession was banned by force of arms, not any constitutional theory. The Civil War essentially imposed a new country and Constitution.

Quote Tweet

Jeremiah “Jasper” Thompson

@jjfThompson

·

Jan 30

There were no other treason trials against former Confederates, because any one trial would have proved the legal right of secession and the Northerners were not about to lose in the courtroom what it won on the battlefield.

>>18300879

no one else thinks China's full mobilization, including all FEMALES 18+, and how it is linked to the balloon isnotable?

>>18301078

>The barroon event

was the signal to the embedded already here, that the Party is starting, IMHO

>>18301054

sauce pls.

o7

>>18301116

this anon has not seen any evidence of white hats outside of those of us posting here, and those who amplify the message and mosaic crafted here.

Warp Speed is a DARPA/DOD Op as is the ongoing PsyOp that enabled and continues to propagate the pandemic state of mind in the masses. MSM is part of the MIC.

guidestones also appeared to be a marker or trigger to next phase actions