Anonymous ID: fb9f94 Jan. 25, 2024, 4:43 a.m. No.20300149   🗄️.is 🔗kun

Asda (UK) confirms almost 100 stores are going cashless - full list of affected locations

 

https://www.express.co.uk/life-style/life/1859573/Asda-cashless-stores-full-list

 

Supermarket chain Asda explained that more than 90 percent of payments are made via card.

Asda 24 Hours Supermarket Entrance Sign

 

Popular supermarket chain Asda has confirmed that nearly 100 of its kiosks will be going card-only, as consumers are moving away from cash transactions.

 

Asda petrol stations are already getting rid of manned tills, with a spokesperson citing the reason being that 90 percent of transactions are made using credit or debit cards.

 

An Asda spokesperson said: "As more than 90 percent of all payments on our superstore forecourts are made via card or a contactless device, the colleagues who worked on these sites are moving into the store so they can better serve our customers.

 

“These conversions began in December and we expect to complete the changeover later in 2024."

 

There are 82 stores making the switch to ‘pay at pump’ only, but the changes are only affecting forecourts that are adjacent to supermarket stores.

Anonymous ID: fb9f94 Jan. 25, 2024, 4:47 a.m. No.20300158   🗄️.is 🔗kun   >>0229 >>0236 >>0573

https://www.tiktok.com/@caseyleebarker/video/7327862546008984875

 

caseyleebarker

☆CASEY BARKER☆

9h ago

 

Texas, America, something is brewing y’all#texasbordercrisis #texassurge #prayerwarriors #spiritualwarfare #christianmotivation #prayer #bible #biblestudy #bibleverse #biblebuild #bibleversedaily #bibleverses #bibletok #christianity #christianitytiktok #christianfyp #endtimes #endtimesigns #endtimesprophecy

Anonymous ID: fb9f94 Jan. 25, 2024, 4:50 a.m. No.20300165   🗄️.is 🔗kun   >>0573

https://twitter.com/MarioNawfal/status/1750376979130470429

 

Mario Nawfal

 

@MarioNawfal

🚨BREAKING: BORDER CIVIL WAR?

 

Several other red states, such as Florida and Oklahoma, have said they will stand by Texas in fighting against Biden's border policies.

 

Federal mandates are enforced by the US Army, BUT the National Guard serves the State.

 

Things are getting concerning…

 

Source: The Politics Brief

All Footage is Not Recent

 

10:36 PM · Jan 24, 202

Anonymous ID: fb9f94 Jan. 25, 2024, 4:51 a.m. No.20300172   🗄️.is 🔗kun   >>0573

https://twitter.com/Bubblebathgirl/status/1750389271846859194?s=20

 

Paul A. Szypula 🇺🇸

@Bubblebathgirl

DeSantis said Florida will send “personnel and assets.”

11:24 PM · Jan 24, 2024

Anonymous ID: fb9f94 Jan. 25, 2024, 4:53 a.m. No.20300177   🗄️.is 🔗kun   >>0187 >>0194 >>0223 >>0573

Wall Street Silver

@WallStreetSilv

Because Texas Gov Greg Abbott has invoked Article 1, Section 10, Clause 3 … other states are now able to enter into agreements with Texas to help repel the invasion that Texas is dealing with.

 

Other states may now send troops and/or equipment to aid in halting in the border invasion.

 

Which states will send troops and equipment to defend the border?

11:11 PM · Jan 24, 2024

 

https://twitter.com/WallStreetSilv/status/1750385861202522130

Anonymous ID: fb9f94 Jan. 25, 2024, 4:58 a.m. No.20300194   🗄️.is 🔗kun   >>0205 >>0256 >>0573

>>20300177

https://twitter.com/ValentinaForSOS/status/1749642762348089417

 

Valentina Gomez

@ValentinaForSOS

I call for the 27 Republican Governors to declare a Unified State of Emergency. Use your Emergency Powers to protect your citizens🚨.

@KristiNoem

 

@GlennYoungkin

@RonDeSantis

@GregAbbott_TX

@SarahHuckabee

@mikeparson

0:01 / 0:24

9:58 PM · Jan 22, 2024

 

https://twitter.com/ValentinaForSOS/status/1749642762348089417

Anonymous ID: fb9f94 Jan. 25, 2024, 5:02 a.m. No.20300208   🗄️.is 🔗kun   >>0209

US Today Show makes awkward King Charles blunder as closed caption reads he is to undergo 'prostitute surgery'

 

https://www.dailymail.co.uk/femail/article-12995571/Today-closed-caption-King-Charles-prostitute-surgery.html

 

The Today Show suffered an awkward blunder on Monday as it's closed captions said King Charles was set to undergo 'prostitute surgery'.

 

The highly-watched US morning show, which airs on NBC, was reporting on the king's recent surgery for an enlarged prostate.

 

But instead of reporting about the medical procedure, the caption read the king was to have 'prostitute surgery'.

 

The blunder was spotted by Below Deck and the Traitors US star Kate Chastain who uploaded it to Instagram and joked 'I bet AI is in charge of closed captions for the Today Show'.

 

Last week the royal was diagnosed with an enlarged prostate and revealed he will attend hospital this week to be treated.

 

The blunder was spotted by Below Deck and the Traitors US star Kate Chastain who uploaded it to Instagram and joked 'I bet AI is in charge of closed captions for the Today Show'.

The blunder was spotted by Below Deck and the Traitors US star Kate Chastain who uploaded it to Instagram and joked 'I bet AI is in charge of closed captions for the Today Show'

Aides said the King's condition was benign and he would be having a corrective procedure. His engagements will be postponed for a short period of recuperation.

 

Queen Camilla has revealed the King is 'fine' as he prepares for the treatment.

 

She has reportedly told 'workaholic' Charles to 'slow down' in the wake of his recent health scare.

 

The Queen, 76, told well-wishers over the weekend that her husband was 'fine' as he awaits an operation for an enlarged prostate.

 

Charles has gained a reputation for being dedicated to his work and packed 2023 full of engagements - with one tally suggesting he undertook 516 over the course of the year including 94 abroad.

 

He had to immediately cancel all public duties on the advice of doctors last week after his symptoms were spotted and he was diagnosed with the benign condition.

 

The Palace said His Majesty would be undertaking 'a short period of recuperation,' which is expected to last around a month. But now, an insider has claimed that the Queen has urged him to take a new approach to his work.

 

The Queen, 76, has told well-wishers that the King, 75, is 'fine' as he awaits an operation after doctors found he has an enlarged prostate

King Charles III looked in good health as he is seen driving on public roads in Sandringham, Norfolk, on 22nd January, 2024

Speaking to The Sun, they said: 'The Queen has told him he needs to slow down a bit.'

 

The King has kept up his workload after he acceded to the throne 16 months ago and had his coronation at Westminster Abbey last May after more than 70 years as heir.

 

Palace aides have worked to ensure that he and Camilla are not overloaded as they adjust to life on the throne.

 

One insider recently suggested that the Queen has struggled with the increased pace of her work.

 

p1

Anonymous ID: fb9f94 Jan. 25, 2024, 5:03 a.m. No.20300209   🗄️.is 🔗kun

>>20300208

 

Meanwhile, the transition is said to have left the King feeling 'anxious', according to another source, cited by The Telegraph.

 

Royal biographer Robert Jobson said that the King is 'a grafter' and 'has always had an innate sense of duty and tried his best to justify his good fortune by working tirelessly to improve the lot of ­others less fortunate than himself'.

 

While Camilla has continued with her back-to-back schedule, the King's public duties are now on hold as he recovers at his Sandringham Estate.

 

His Majesty will be admitted to an unidentified hospital to be treated this week.

 

Queen Camilla leaves after a visit to a women's refuge on January 22, 2024 in Swindon

A young girl holds a get well card for King Charles III as she waits to greet Queen Camilla during her visit to Deacon & Son Jewellers in Swindon, western England on January 22, 2024

His Majesty speaks to members of the public ahead of the Sunday church service at St Mary Magdalene Church on January 7

The Royal family was hit by a double health woe with it also being announced that the Princess of Wales had abdominal surgery (Pictured with Charles in 2022)

Camilla also said last week that the King was 'fine', adding that he is 'looking forward to getting back to work' despite the shock diagnosis.

 

In the 2018 BBC documentary Prince, Son and Heir, Prince William said: 'The man never stops.

 

'When we were kids there were bags and bags and bags of work that the office just sent to him.'

 

Prince Harry also shared the feeling that their father should 'slow down' a bit.

 

Announcing Charles' diagnosis last week, a Buckingham Palace spokesman said : 'In common with thousands of men each year, The King has sought treatment for an enlarged prostate.

 

'His Majesty's condition is benign and he will attend hospital next week for a corrective procedure. The King's public engagements will be postponed for a short period of recuperation.'

 

The King and Queen Camilla were at his Scottish home Birkhall when they received the shock news, just after it was disclosed that the Princess of Wales was in hospital

 

2 of 2

Anonymous ID: fb9f94 Jan. 25, 2024, 5:04 a.m. No.20300219   🗄️.is 🔗kun   >>0246

https://twitter.com/TubaPhat/status/1750487866927374738

 

~o00O00oo; )

@TubaPhat

Bioluminescence. The hallmark of being a chimera after successful bioterrorism using mRNA.

5:56 AM · Jan 25, 2024

Anonymous ID: fb9f94 Jan. 25, 2024, 5:09 a.m. No.20300246   🗄️.is 🔗kun   >>0269

>>20300219

https://www.chem-agilent.com/pdf/5988-4320EN.pdf

 

==Monitoring transfection efficiency by

green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer==

 

Abstract

This Application Note describes how the Agilent 2100 bioanalyzer and the Cell Fluorescence LabChip® kit can be used to determine the efficiency of transfection of mammalian cells using green fluorescent protein (GFP) as a reporter molecule. Transfection of CHO-K1 cells with expression vectors encoding GFP from two different sources, and optimization of transfection conditions for both plasmids were performed.

Histogram quality and the percent transfected cells determined, based on the low number of fluorescent cells counted with the microfluidic

system, is in good agreement with data obtained with a conventional flow cytometer. Detailed protocols and reagent recommendations for analyzing transfection reactions are given. High reproducibility of chip results, low cell consumption and ease-of-use are advantages the compact Agilent 2100 bioanalyzer offers for monitoring transfection efficiency using GFP.

Anonymous ID: fb9f94 Jan. 25, 2024, 5:11 a.m. No.20300260   🗄️.is 🔗kun

Non-Viral Transfection Methods Optimized for Gene Delivery to a Lung Cancer Cell Line

Loghman Salimzadeh,1 Mansooreh Jaberipour,1 Ahmad Hosseini,1 and Abbas Ghaderi1,2,*

Author information Article notes Copyright and License information PMC Disclaimer

Go to:

Abstract

Background

Mehr-80 is a newly established adherent human large cell lung cancer cell line that has not been transfected until now. This study aims to define the optimal transfection conditions and effects of some critical elements for enhancing gene delivery to this cell line by utilizing different non-viral transfection Procedures.

 

Methods

In the current study, calcium phosphate (CaP), DEAE-dextran, superfect, electroporation and lipofection transfection methods were used to optimize delivery of a plasmid construct that expressed Green Fluorescent Protein (GFP). Transgene expression was detected by fluorescent microscopy and flowcytometry. Toxicities of the methods were estimated by trypan blue staining. In order to evaluate the density of the transfected gene, we used a plasmid construct that expressed the Stromal cell-Derived Factor-1 (SDF-1) gene and measured its expression by real-time PCR.

 

Results

Mean levels of GFP-expressing cells 48 hr after transfection were 8.4% (CaP), 8.2% (DEAE-dextran), 4.9% (superfect), 34.1% (electroporation), and 40.1% (lipofection). Lipofection had the highest intense SDF-1 expression of the analyzed methods.

 

Conclusion

This study has shown that the lipofection and electroporation methods were more efficient at gene delivery to Mehr-80 cells. The quantity of DNA per transfection, reagent concentration, and incubation time were identified as essential factors for successful transfection in all of the studied methods.

 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689559/

Anonymous ID: fb9f94 Jan. 25, 2024, 5:13 a.m. No.20300269   🗄️.is 🔗kun   >>0290

>>20300246

Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer

 

recall all of the blue/purple street lights that when up in 2020-2022?

Anonymous ID: fb9f94 Jan. 25, 2024, 5:31 a.m. No.20300344   🗄️.is 🔗kun   >>0361 >>0573

Iran, Turkey sign 10 cooperation documents

 

https://en.mehrnews.com/news/211272/Iran-Turkey-sign-10-cooperation-documents

 

Turkish President Recep Tayyip Erdogan met his Iranian counterpart Ebrahim Raeisi in Ankara on Wednesday.

 

In the presence of the two presidents, Iranian and Turkish high-ranking officials including ministers signed 10 cooperation documents.

 

These documents of cooperation were inked in the field of railways, energy exchange, and establishment of joint commercial and communication zones.

 

Raeisi and Erdogan held a meeting in the Turkish presidential palace before attending the signing ceremony.

 

Before their one-on-one meeting, Erdogan welcomed Raeisi in an official ceremony at the presidential palace.

 

Speaking in a joint press conference with the Turkish president after their meeting, the Iranian president reiterated that the United Nations has lost its effectiveness regarding the Israeli regime’s genocidal war against Palestinians in Gaza.

 

Raeisi stressed that those who have committed crimes against the Palestinians must be held accountable.

 

The Iranian president said that Palestine is the most important issue of the Islamic world and humanity, adding that the United States is responsible for the Israeli atrocities in Gaza because of its support of the regime.

 

Raeisi said that regional cooperation was the only solution to address terrorism in the region. “Iran-Turkiye cooperation in the fight against terrorism is of great importance,” he added.

 

The president said Iran considers the security of Turkey and other regional countries as its own security.

 

“We are determined to combat terrorism for the security of Turkey and the countries in the region,” he noted.

 

Raeisi, heading a political and economic delegation to participate in the eighth meeting of Iran-Turkey Supreme Council of Economic Cooperation, arrived in Ankara earlier on Wednesday and was received by President Erdogan.

 

MNA

Anonymous ID: fb9f94 Jan. 25, 2024, 5:39 a.m. No.20300383   🗄️.is 🔗kun   >>0390

>>20300361

indeed.

> can’t control long time allies

doesn't 'our greatest ally' blackmail and extort our 'laymakers' and presidents with OPs like Epstein and Maxwell (father and daughter(s)?

 

Ally means something different in dictionary vs practice/reality.

 

Allies seem to be one side has dirt, the other does bidding.

Anonymous ID: fb9f94 Jan. 25, 2024, 5:45 a.m. No.20300406   🗄️.is 🔗kun   >>0411

>>20300321

yet the military/DARPA contracted the mrna gene editing tech that has killed, maimed, and damaged so many.

 

each day more vaxxed realize what has been done, and they dig a bit, they will see their mil, gov, presidents, cdc, fda, nih, and media knowingly did this to them. And that the main capable force to prevent it, the mil, was the key driver of it, since at least 2013 when DARPA ordered the mrna tech from moderna, pfizer, etc.

 

trust in the mil is not likely IMHO, and rightfully so.

Anonymous ID: fb9f94 Jan. 25, 2024, 5:50 a.m. No.20300422   🗄️.is 🔗kun   >>0434

>>20300134

predictive programming in media and major events have him and his family name going back to the 1934 and 1939 Worlds Fairs, as well as comic books, TV shows and movies from the 50s.

https://variety.com/2019/politics/news/trackdown-1950s-tv-show-border-wall-trump-1203106000/https://variety.com/2019/politics/news/trackdown-1950s-tv-show-border-wall-trump-1203106000/

 

Heavy metal magazine in the 1980s, etc etc

 

Well documented in playlists by @enterthestars on youtube.

Anonymous ID: fb9f94 Jan. 25, 2024, 5:57 a.m. No.20300451   🗄️.is 🔗kun   >>0462 >>0464 >>0502

>>20300434

>>20300434

>time travel

 

#19890338 at 2023-11-10 01:36:09 (UTC+1)

Q Research General #24422: E-Bake Seeking Passing Baker Edition

 

>>19890239

"Time travel is fun" Q 3555

 

https://golfweek.usatoday.com/2022/07/31/why-delorean-car-time-machine-from-back-to-the-future-liv-golf-trump-bedminster/

 

same place ex-wife was buried… golf course

 

And dont forget Biff Tannen was based on Trump, per the writers…

 

https://www.nme.com/news/back-to-the-future-writer-reveals-biff-tannen-was-inspired-by-donald-trump-2560286

 

https://www.huffpost.com/entry/trump-is-biff-tannen_n_5627978be4b0bce347031f84

 

https://www.independent.co.uk/arts-entertainment/films/news/michael-j-fox-trump-biff-back-to-the-future-b1760681.html

 

https://www.rollingstone.com/politics/politics-news/back-to-the-future-writer-biff-is-donald-trump-190408/

 

AND Gremlins 2 is based on Trump

 

https://nypost.com/2015/08/19/that-time-donald-trump-was-parodied-in-gremlins/

 

https://www.wired.com/2016/03/gremlins-trump/

 

"presidential hopeful with a character called Daniel Clamp"

Warner Bros. had promised Dante full creative control for part two, and while he had a setting (New York) and a storyline (Gremlins getting Gremlinish in a super-smart midtown building), he needed inspiration for the movie's villain, a vain and wealthy real estate developer with a carefully tamed coif and a brash Manhattan manor. "At that time in New York City," remembers Dante, "there was one major character who was Mr. Billion"—Donald Trump.

 

Which is how Dante-with co-screenwriter Charlie Haas (Over the Edge) and producer Michael Finnell-cooked up the character of Daniel Clamp (played by John Glover), a suit-clad bajillionaire who has a foothold in everything from sports teams to construction companies to jams and jellies, and whose own buildings hawk copies of his very Trump-like autobiography.

 

Though the character wasn't wholly based on the Donald-in a nod to Ted Turner, Clamp also owns a vast, niche-focused cable network-his merchandising-mad mindset and man-about-town demeanor is clearly indebted to Trump's end-of-the-decade persona. As the '80s came to a close, Trump was famous enough to have earned his own board game and a spot alongside the Ramones and Rick Moranis in a primo Bobby Brown video. Yet he also was under increased scrutiny in his hometown, most notably from Spy magazine, which famously labeled Trump a "short-fingered vulgarian" and questioned his financial dealings.

 

At the time, Trump was "overbearing and obviously kind of goofy," says Haas. "He was an emblem of what was going on in the '80s and '90s with greed and money and crassness, and [the idea of] the whole world being for sale. But he still seemed sort of harmless."

 

"Who knew? We never imagined he'd run for president," says director Joe Dante, who parodied Donald Trump as a major character 25 years ago in the horror comedy sequel "Gremlins 2: The New Batch."

 

John Glover receives third billing as "Daniel Clamp," a wacky, publicity-loving real estate developer and best-selling author who wants to level part of downtown New York to create the "Clamp Chinatown Center, where business gets oriented."

 

Much of the action takes place at the mogul's brand-new "Clamp Premier Regency Trade Center" in Midtown - obviously modeled on the Trump Tower on Fifth Avenue, which opened in 1983 - where the logo outside shows the world held in a vise.

 

"It was pretty obvious from the name who we were talking about," says Dante, "though Clamp is also part Ted Turner, since he runs a cable empire as well. Clamp was originally supposed to be the megalomaniac villain of the piece, but he was so oddly endearing, he ended up becoming a semi-heroic figure."

Anonymous ID: fb9f94 Jan. 25, 2024, 6:21 a.m. No.20300536   🗄️.is 🔗kun

>>20300526

 

The position of the pyramids in the Shensi China area:

  1. 34° 26' 42" N / 108° 56' 25" E

  2. 34° 26' 39" N / 108° 56' 00" E

  3. 34° 26' 00" N / 108° 52' 36" E

  4. 34° 26' 05" N / 108° 52' 12" E

  5. 34° 25' 18" N / 108° 50' 12" E

  6. 34° 23' 25" N / 108° 44' 12" E

  7. 34° 24' 00" N / 108° 42' 30" E

  8. 34° 22' 28" N / 108° 41' 35" E

  9. 34° 21' 40" N / 108° 38' 10" E

  10. 34° 20' 15" N / 108° 34' 00" E

  11. 34° 10' 45" N / 109° 01' 12" E

  12. 34° 10' 41" N / 109° 01' 25" E

  13. 34° 10' 37" N / 109° 01' 38" E

  14. 34° 14' 15" N / 109° 05' 42" E

  15. 34° 14' 05" N / 109° 07' 00" E

  16. 34° 23' 00" N / 109° 15' 00" E

Anonymous ID: fb9f94 Jan. 25, 2024, 6:33 a.m. No.20300593   🗄️.is 🔗kun

>>20300580

yes.

AND when you know you work a 50+ year career with pedophiles, cannibals, blackmailers, ritual practitioners, and murderers, then you know you could and should share this via your social media platform, but choose not to, what's that called?