I was on the blast site and I finally found the freaking ability to check exactly what strand they are referring to when they show me the homology.

I will be rechecking some of these primers that I have in the 5>3 direction

I don't actually have anything, just sequence AI gave me

I am hoping to be able to find segments that have mismatched base pairs within/// while full length of match remains unchanged.

For instance, AAAAAAAAAAA

ACCACAAAAAC

How do I accomplish this search?

>>20450661

*mismatched similar sequences