Pole Shift, etc now has a published window of likely initiation…

For those familiar, this is the scenario, the AMOC collapse, depicted in the Day After Tomorrow.

==Machine-learning prediction of tipping and collapse of the Atlantic Meridional

Overturning Circulation==

Recent research on the Atlantic Meridional Overturning Circulation (AMOC) raised concern about its potential collapse through a tipping point due to the climate-change caused increase in the freshwater input into the North Atlantic. The predicted time window of collapse is centered about the middle of the century and the earliest possible start is approximately two years from now. More generally, anticipating a tipping point at which the system transitions from one stable steady state to another is relevant to a broad range of fields. We develop a machine-learning approach to predicting tipping in noisy dynamical systems with a time-varying parameter and test it on a number of systems including the AMOC, ecological networks, an electrical power system, and a climate model.For the AMOC, our prediction based on simulated fingerprint data and real data of the sea surface temperature places the time window of a potential collapse between the years 2040 and 2065.

https://export.arxiv.org/pdf/2402.14877

https://export.arxiv.org/abs/2402.14877

>>20478377

and king Charles

and pope Francis and his cadre of pedo gangster bishops and cardinals

and Antonio Guterres

and Agustín Carstens

and François Villeroy de Galhau

and a few others, but yeah

Expert on Squatters Warns Nation: We're in for a 'Nightmare' Once Illegal Immigrants Discover Housing Law Loopholes

https://www.westernjournal.com/expert-squatters-warns-nation-nightmare-illegal-immigrants-discover-housing-law-loopholes/

America’s housing law loopholes are just big enough for illegal immigrants to crawl through, according to one expert who warns that tenants’ rights laws make it hard to dislodge squatters.

“We have masses coming in. They’re going to be looking for places to live. And if we don’t have the housing for them, if they’re coming in with no money, they can’t rent the traditional way,” Flash Shelton, founder of the United Handyman Association and SquatterHunters.com, said, according to Fox News.

Shelton used innovative tactics to dislodge squatters from his mother’s house in 2019, and has emerged as an advocate to help others facing the difficult chore of ridding a property of people who choose to remain.

The map of tenants’ rights laws is varied, because it is a local or state issue. Many states require a civil suit process to evict someone, which is burdensome and time-consuming.

Once illegal immigrants get wind of the rights they have as squatters, “our squatter situation is going to go beyond control,” Shelton said.

Shelton noted that squatters are already emboldened by local laws, adding, “What are we going to do later when we have a million people squatting in this country?”

Shelton said the housing picture will grow worse “when, not only do we have a border issue that we can’t even figure out, but now we have people that are being mentally, financially, physically messed with because they’ve lost their home to all of these people?”

“Regardless of how you feel politically or morally about the situation, put that aside and just think about the masses,” Shelton said.

Almost 7.3 million illegal immigrants have entered the U.S. since the beginning of 2021, according to Fox News.

Should squatting be illegal?

Yes No

Completing this poll entitles you to The Western Journal news updates free of charge via email. You may opt out at anytime. You also agree to our Privacy Policy and Terms of Use.

“We have irresponsibly opened a door for a whole lot of people to come into this country, and we aren’t prepared to deal with them,” Shelton said.

“What’s the negative for these people to then start taking over these houses?” he said.

Shelton predicted a “nightmare scenario” without laws that crack down on squatters.

“I’m spending all this time trying to bring awareness to squatting and bring awareness to how the law needs to change,” Shelton said. “Is it going to get worse before it gets better? I think it’s already there.”

“I think it’s going to just get beyond repair at some point,” he said.

Atlanta is already reeling with an estimated 1,200 homes taken over by squatters, according to Fox News. Shelton said making squatting a terrorist act might be the next step needed to regain control.

“If we were to criminalize it, that would enable us to send in the National Guard to sweep that whole neighborhood and get those 1,200 houses clear,” he said.

Shelton has posted a petition on Change.org to support making squatting illegal. He also set up a GoFundMe appeal that has raised more than $8,000 toward the cause.

https://twitter.com/BGatesIsaPyscho/status/1761338276453978144

Concerned Citizen

@BGatesIsaPyscho

🚨🇬🇧 London, UK

More shops are requiring a QR code simply to gain access in order to buy food - this is happening NOW.

The Covid Scam allowed for Digital infrastructure to be installed for next stage of Agenda 2030.

Digital ID & CBDC are coming, both will be mandatory for people to simply interact in society.

Next comes a Co2 Tracker & Social credit score - then guess what - we are the same as communist China.

Do you get any say in this? Of course you don’t.

4:32 AM · Feb 24, 2024

Truth Slinger X

@TruthSlingerX

Did she really say this OUT LOUD????

0:11 / 2:41

8:33 AM · Feb 25, 2024

https://twitter.com/TruthSlingerX/status/1761761319240302999

>>20478097

>>20478494

>>20478471

>ENOU[G]H IS EN[O]UGH.

[G] [O]

GO

>>20478424

and no one wants to acknowledge…

smh

Kevin McKernan has found spike->human DNA integration events using human cancer cells + mrna jabs

https://anandamide.substack.com/p/vaccine-targeted-qpcr-of-cancer-cell

Vaccine targeted qPCR of Cancer Cell Lines treated with BNT162b2

Putative integration events

Ulrike Kaemmerer has treated MCF7 and OvCar3 cancer cell lines with various vaccines. Once transfected they performed cell passaging on these transfected cell lines to dilute out the residual vaccine and identify cells which were transfected. They performed Immunohistochemistry (IHC) on these cells and documented spike expression levels.

They then reached out to us to see if we could find any DNA or RNA in the cell lines. This thread will document what we found from a DNA and RNA level.

Staining protocol given in (Mörz M. A Case Report: Multifocal Necrotizing Encephalitis and Myocarditis after BNT162b2 mRNA Vaccination against COVID-19. Vaccines (Basel). 2022 Oct 1;10(10):1651. doi: 10.3390/vaccines10101651. PMID: 36298516; PMCID: PMC9611676.)

Below is a description of the qPCR and sequencing performed.

When assessing genome integration events one has to consider the potential rare side reactions that occur with Illumina sequencing. Illumina makes use of an End Repair step that adds an A to each 3’ end of dsDNA to create a Adenosine overhang DNA. This is known as a "“Plus A” reaction usually performed with a Klenow polymerase that tends to add As to 3 hydroxyl ends of blunt dsDNA.

Nepetalactone Newsletter is a reader-supported publication. To receive new posts and support my work, consider becoming a free or paid subscriber.

This step is critical to ensure gDNA doesn’t ligate to itself and make chimeric DNA during ligation reactions. The sequencing primers are then added in excess. These primers have a Plus T on them. This ensures that only sequencing primer can ligate to gDNA and genomic DNA will be incompatible with self ligation. If for some reason the Plus A reaction doesn’t go to completion and some gDNA fails to Plus A, chimeric gDNA can ligate. This chimera formation side reaction should be random thus its highly unlikely for 2 fragments to ligate to each other at the exact same base in the genome. The odds of getting 2 fragments to form chimeric molecules at the same base in the genome is a 1 in 3 billion chance.

The way to combat this is to require at least 2 different unique molecules to call an integration event.

What is a ‘unique’ DNA molecule?

p1

>>20478595

Each DNA fragment has a defined start and stop base. DNA molecules that have the same start and stop base can occur naturally in the DNase step used to break up the DNA into small pieces for Illumina sequencing. These duplicate sequences are often discounted as they could also arise with the PCR process used to amplify the Illumina library. These are called PCR duplicates or ‘dupes’. Tools like Picard look for reads that share the same start and stop base and flag them as potential PCR duplicates. The Illumina sequencers can also make duplicate sequences with what are known as optical duplicates. This is a software artifact that calls a single colony on the sequencing array as two colonies. As a result these sequencing reads also have the same start and stop base. They are easier to identify as their coordinates on the array are in close proximity.

There are techniques developed in the field to address this using UMIs (Unique molecular identifiers). UMIs are synthetic DNA barcode sequences you ligate on the ends of your DNA before any amplification is performed. Since every DNA molecule gets a unique barcoded added to it, you can discern if reads that share the same start and stop base also have the same DNA barcode. If they do then you know PCR over amplified that molecule. You can then use these UMIs to normalize the differential PCR that may have occurred when amplifying a library. Some regions may amplify 1,000 X and others 10,000X and this will be easy to sort out by only collecting the same amount of sequence from each UMI barcode. In our experiment we did not use UMIs so we must treat all reads with the same start and stop point as non-unique reads.

For these reasons, we do not take any singleton read as evidence for an integration event. We want to see at least 2 unique molecules with different start and stop bases covering a chimeric vaccine sequence to human sequence event to consider this a putative integration event.

Since sequencing can be costly, we first screen which cells are worth sequencing by qPCR screening the cells after vaccine treatment. Only qPCR positive cells will be graduated to sequencing.

We first start with qPCR of the vaccines before they are applied to cells to understand the quantity of the DNA contamination.

qPCR of the some of these mRNA vaccines record CT scores as low as CT 12.72!

qPCR methods were described previously.

Table 1: qPCR of the vaccine only

1:10 dilutions are performed as in some vaccine vials we have noticed that direct qPCR of the vaccine itself inhibits qPCR.

Table 2 depicts 1:10 dilutions in qPCR of cell lines treated with various vaccines. OVCAR is OvCar3. P1 and P2 represent the cell passage that has occurred. P1 = 1st passage, and P2 = 2nd passage. Mod = Moderna Lot (133 vs 140A). PB = Pfizer/Biontech with lots (9715 and 1042A).

RNAP is an internal control that amplifies a human genome. This primer set is off the shelf from IDT as it is used in C19 qPCR.

Table 2- 1:10 Dilution of treated cells into qPCR

Note Moderna is not expected to amplify with SV40. The NTCs are important to pay attention to. A few NTCs are lighting up in the 37-38 range. This tells you that you shouldnt trust any data from those channels past that CT score. The bacterial Origin of replication is known to always amplify the NTCs at CT 37+ because the polymerase used in these qPCR kits was expressed from a bacterial plasmid that has this Ori and small amounts of this residual DNA can be detected out at CT 37. There is one SV40 positive in Moderna at 36.90 while the NTCs are all clean for SV40. This could a result of SV40 having a low level contamination in those cell lines. Note this is 3-5 CTs later than where Pfizer SV40 is detected so it may just be noise.

RT-qPCRthe vaccine lots are the combination of RNA and DNA signals and present even lower CT scores for SV40 (Table 3). This was RT-qPCR of the vaccines directly BEFORE they were applied to the cell lines listed.

p2

>>20478598

Table 4 is RT-qPCR of the cells after the vaccine was applied to each cell line and passaged. You’ll note Passage 1 is ~5 CTs earlier than Passage 2. We don’t expect RNAP to differ between passages. Notice the vector amplicon is CT 30.28 on the 1st passage for OVCARP1 and 34.72 for the 2nd passage. ~4CTs is 16 fold difference.

Since several 100 fold dilutions (10ml washes with PBS) were performed between passages, if this were simple dilution between passages we would expect 100 -10,000 fold reduction in CT scores which is 6-12 CTs. We don’t see this which demonstrates the DNA/RNA is in the cells and not diluting out with each wash.

The qPCR data shows a larger delta CT between passages (5-6CTs or 32-64 fold dilution).

Table 4. RT-qPCR of cells after vaccine treatment.

Rerun at 1:10 Dilution in RT-qPCR

Watchmaker DNA libraries were constructed according to the manufacturers protocol for Passage 1 and Passage 2 qPCR positive OVCAR cell lines. 100ul of cells were lysed with MaGiC Lysis (Medicinal Genomics part number P/N 420180) and used for Watchmaker library construction. Sequencing libraries were DNA barcoded and sent out to a blinded Illumina sequence provider

Sanger Sequencing of PCR products derived from the Illumina Library designed to capture the integration event.

Sanger verification of the integration event in the Illumina Library is seen above.

Additional observations

The depth of sequencing over the vaccine in OvCar3 is over 3,000X while the depth of sequencing over the OvCar3 genomes is 30X. As a comparison, when sequencing a diploid human genomes (2X coverage) you usually get 1,000-10,000X coverage for the mitochondrial genome as it is multi copy in each cell. A similar thing is seen in plants that have both mitochondria and chloroplast genomes. These vaccine plasmids are at 100X coverage per 1X human genome. That is higher than I expected and far higher than any fact checker predicted.

Another odd thing is observed. There are SNPs in the plasmid vector that do not appear in the vaccine that never sees any cells. This is a sign that the cells are mutating the plasmids.

Why are there SNPs in the plasmid F1 Origin only when the plasmid is transfected into a cell? These SNPs are not present in the vaccine control sequence (Left side of bottom track in above figure). The variants are in phase and are 20% in allele frequency.

p3

>>20478610

The F1 Origin is derived from a Phagemid which initiates ssDNA synthesis. They are known to form hairpins.

The variants are in the stem loop of the F1 Ori highlighted in yellow.

There is a forth SNP in the OvCar3 vaccine sequence at the 3’ end of this highlighted stretch in the SV40 Origin (GAGGC[C/G]). This is at 40% allele frequency.

Given all 4 of these variants exist in Origins of replication, this is likely evidence that these components are being replicated in OvCar3 cells lines and these are replication errors. This is supported by the higher than average sequence coverage over these regions of the OvCar3/vaccine sequence. Note the SNPs fall on coverage peaks in the IGV coverage plots below. The replication is unlikely to span large distances as most of the fragments are short in length but some of the larger fragments observed with oxford nanopore sequencing of the vaccine could be readily amplified to higher levels once inside the cell. Some of these 3kb fragments contained the entire Neo/Kan gene with its SV40 promoter and SV40 Ori.

Discussions and Limitations

This is a cancer cell line that doubles every 60 hours. This doesn’t imply that this is happening in patient cells but it may be a model for what a cancer patient in remission may face upon vaccination.

There are several limitations to this study.

1)While we have Sanger sequence confirmation of these chimeric events in the Illumina sequencing library that was generated, we consumed most of the cells to make that sequencing library and cannot confirm this is in the remnant cells we have. Future studies will culture larger quantities of cells to afford more retrospective scrutiny of these events in the original vaccinated cell line. These are rare events so they are unlikely to be present in all cells and resampling a different collection of cells from the same batch of cells may not capture the same events.

2)Since we used short reads from ILMN, we do not have confirmation of both ends of the integration event. Thus we cannot opine on the size of the integration event until we capture the other end of the integration event. Deeper sequencing of the Illumina library may find this event and the use of longer reads (ONT or PacBio) should be explored in future studies. These platforms require more DNA input and may require a scaled up transfection (more cells).

3)Both (Chr9 and Chr12) putative integration events are supported by multiple reads in the library. Singleton events cannot be trusted as they could be the result of chimeric ligation in the Illumina library ligation step. The odds of two molecules in the library sharing the same integration point as a function of random chimera formation in library construction is 1 in 3 billion likelihood. Having this occur twice (Chr9 and Chr12) in the vaccinated cell lines with 2 independent reads supporting each event and zero times in the unvaccinated control implies these are strong contenders for integration. Sanger sequencing confirmation of amplicons traversing the integration event using different primers than used on the Illumina cluster station, implies these are not artifacts of cluster PCR, index hopping or optical artifacts known to occur at low frequencies on the Illumina sequencers.

4)Cancer cell lines are known to go through Chromothripsis which is a shattering of the genome. It is possible these chimeric events represent ligation to fragments of chromosomes which are extrachromosomal. Therefore long reads are required to resolve more ephemeral chromothripsis events from longer term chromosomal integration.

In summary, this data does demonstrate that SV40, Origin of replication and spike DNA can be found through at least 2 cell passages in vaccinated OvCar3 cell lines. This is not seen in the untreated cell lines. Additionally spike DNA was detected in Whole Genome Shotgun libraries of the vaccine treated samples at 3,000X coverage while the human genome was only at 30X coverage. Further work is required to validate the size and frequency of these integration events and rule out Illumina related artifacts that could lead to false positive integration events.

p4

>>20478616

Additionally, detection of SNPs in the vaccine plasmids origin of replications (F1 and SV40) is a sign that OvCar3 cells lines are initiating replication of DNA once transfected. Thus small amounts of DNA contamination containing superfluous mammalian origins of replication can result in larger quantities of DNA once inside a cell. This should inform regulations regarding residual DNA contamination. A simple 10ng limit on all DNA should be reconsidered if the contaminating DNA contains billions of copies of mammalian origins of replication. The Moderna vaccines do not contain SV40 origin or F1 origin thus it is obvious these sequences are unnecessary risks in the Pfizer vaccine.

This work has taught us that several regions of the plasmid DNA that are identical to human mitochondrial sequences or polyA sequences cannot be ascertained for integration as they exist in both the vaccine and the human genome.

Further work is required to validate these putative integration events. Longer reads and deeper sequencing are now justified and should be prioritized.

FastQ Sequencing Data for OVCAR3-PB9715

•https://mega.nz/file/9ZgyjYrQ#zZUf8X5FkW-a2_sGBN4G37TqQkLFITMQf77_-Uq0ZAo

•https://mega.nz/file/VRQwyAiT#LQGQCjNv3YNqCImrH8ENJGdek5qxphGXS6xTZucAMII

If your browser asks for an encryption key, try another browser. The encryption key is after the # in the URL but some browsers don’t parse this.

5 of 5