The Cessna up for grabs is painted red, white and blue and registered N529JK — in honor of President Kennedy's May 29 birthday.
It has recently undergone a "major engine upgrade" and is ready to fly, according to Leland's.
Bidding will begin online at lelands.com on Nov. 10 and conclude on Dec. 2.
Leland's spokesman Marty Appel said the plane's owner did not want to be identified — but insisted the auction was not trying to cash in on tragedy.
"This is a piece of history. It's of historical interest to people," Appel said.
He said the 1977 Cessna model would usually sell "in the low six figures — $100,000 to $120,000" without the JFK name behind it.
JFK Jr., a former Manhattan assistant district attorney, had been editor of George magazine when he died.