Maybe this is why so many people are screwed up in their sexual preferences, gender determination, and sexual orientation. It's not just the XX or XY chromosomes that are involved. There are regulator genes (aka enhancer genes) that increase or decrease the activity of other genes which decide if we become male or female. For example, if the SOX9 gene is disrupted in an XY embryo, then the testes won't develop properly. I think this is where endocrine disrupters in plastics, foods, etc. are having an effect in our kids and subtly (or perhaps very obviously) changing them. If your regulator genes are disrupted, then a boy won't "feel" like a boy and vice versa for girls.
https://medicalxpress.com/news/2018-12-geneticists-discovery-baby-sex.html
Geneticists make new discovery about how a baby's sex is determined
December 14, 2018, University of Melbourne
Medical researchers at Melbourne's Murdoch Children's Research Institute have made a new discovery about how a baby's sex is determined—it's not just about the X-Y chromosomes, but involves a 'regulator' that increases or decreases the activity of genes which decide if we become male or female.
The study, 'Human Sex Reversal is caused by Duplication or Deletion of Core Enhancers Upstream of SOX9' has been published in the journal Nature Communications. MCRI researcher and Hudson Institute Ph.D. student, Brittany Croft, is the first author.
"The sex of a baby is determined by its chromosome make-up at conception. An embryo with two X chromosomes will become a girl, while an embryo with an X-Y combination results in a boy," Ms Croft said.
"The Y chromosome carries a critical gene, called SRY, which acts on another gene called SOX9 to start the development of testes in the embryo. High levels of the SOX9 gene are needed for normal testis development.
"However, if there is some disruption to SOX9 activity and only low levels are present, a testis will not develop resulting in a baby with a disorder of sex development."
Lead author of the study, Professor Andrew Sinclair, said that 90 percent of human DNA is made up of so called 'junk DNA or dark matter' which contains no genes but does carry important regulators that increase or decrease gene activity.
"These regulatory segments of DNA are called enhancers," he said. If these enhancers that control testis genes are disrupted it may lead to a baby being born with a disorder of sex development."
Professor Sinclair, who is also a member of the Paediatrics Department of the University of Melbourne, said this study sought to understand how the SOX9 gene was regulated by enhancers and whether disruption of the enhancers would result in disorders of sex development.
"We discovered three enhancers that, together ensure the SOX9 gene is turned on to a high level in an XY embryo, leading to normal testis and male development," he said.
"Importantly, we identified XX patients who would normally have ovaries and be female but carried extra copies of these enhancers, (high levels of SOX9) and instead developed testes. In addition, we found XY patients who had lost these SOX9 enhancers, (low levels of SOX9) and developed ovaries instead of testes."
Ms Croft said human sex reversal such as seen in these cases is caused by gain or loss of these vital enhancers that regulate the SOX9 gene; consequently, these three enhancers are required for normal testes and male development."
"This study is significant because in the past researchers have only looked at genes to diagnose these patients, but we have shown you need to look outside the genes to the enhancers," Ms Croft said.
Professor Sinclair said that across the human genome there were about one million enhancers controlling about 22,000 genes.
"These enhancers lie on the DNA but outside genes, in regions previously referred to as junk DNA or dark matter," he said. "The key to diagnosing many disorders may be found in these enhancers which hide in the poorly understood dark matter of our DNA."
Journal article: Brittany Croft et al, Human sex reversal is caused by duplication or deletion of core enhancers upstream of SOX9, Nature Communications (2018). DOI: 10.1038/s41467-018-07784-9
https://www.nature.com/articles/s41467-018-07784-9