Attached PDF is from 2010…

State Key Laboratory of Virology, Wuhan Institute of Virology,

Chinese Academy of Sciences (CAS), Wuhan 430071, Hubei,

China

An HIV-1-luciferase pseudotype virus carrying the SARS-

CoV BJ01 S protein, HIV/BJ01-S, was prepared as

described previously [13]. HeLa cells were seeded onto

96-well plates for 18 h and then transfected with 0.2 lg

recombinant plasmid containing bat or human ACE2 using

0.5 lL Lipofectamine 2000 (Invitrogen, USA) according to

the manufacturer’s protocol. At 24 h post-transfection,

30 lL medium containing HIV/BJ01-S was added to each

well.

To examine the susceptibility of different bat ACE2 mol-

ecules to SARs-CoV entry, the HIV/BJ01-S pseudovirus

system was used to infect HeLa cells transiently expressing

bat ACE2 or human ACE2 genes