tyb
ty anon!!!!
Austrailian AF R8889 out of Shanghai-very little information available on this AC except for regional flights in Oz
Ran the coordinates to check and see if it was just an anomaly and this is what showed up…Cap#2-from Google Patents
Method for designing mutated enzyme, method for preparing the same and mutated enzyme
The present invention provides a novel method for improving an enzyme hydrolyzing an alpha-1,6-glycosidic linkage. A mutated enzyme is designed by specifying one or more amino acids selected from the group shown below in an amino acid sequence of an enzyme (an enzyme to be mutated) that hydrolyzes an a-1,6-glycosidic linkage, that is, the group consisting of an amino acid corresponding to an amino acid at the 292 position, an amino acid corresponding to an amino acid at the 371 position, an amino acid corresponding to an amino acid at the 406 position, an amino acid corresponding to an amino acid at the 407 position, an amino acid corresponding to an amino acid at the 437 position, an amino acid corresponding to an amino acid at the 465 position, an amino acid corresponding to an amino acid at the 475 position, an amino acid corresponding to an amino acid at the 476 position, an amino acid corresponding to an amino acid at the 525 position, an amino acid corresponding to an amino acid at the 526 position, an amino acid corresponding to an amino acid at the 580 position and an amino acid corresponding to an amino acid at the 582 position of the amino acid represented by in SEQ ID NO: 2 (step (1)) and constructing an amino acid sequence in which the amino acid(s) specified in the step (1) is/are substituted with another amino acid or deleted based on the amino acid sequence of the enzyme to be mutated (step (2)) The design method of mutated enzyme, preparation method and mutated enzyme
Technical field
The present invention relates to design method, preparation method and the mutated enzyme etc. of the mutated enzyme of hydrolyzing alpha-1,6 glycosidic link.
Background technology
Starch debranching enzyme (EC 3.2.1.41) is the enzyme of α-1,6 glycosidic link of pulullan in the hydrolyzed starch etc.Starch debranching enzyme is the high enzyme of usability on the production of Fructus Hordei Germinatus oligoses such as glucose, SANMALT-S, trisaccharide maltose, maltotetrose, maltopentaose, MALTOHAXAOASE sugared fields such as (OLIGOSACCHARIDES, Gordon and Breach Science Publishers, p3), the improvement (patent documentation 1) of cooking etc., industry.
moar at link
https://patents.google.com/patent/CN101528923B/en
https://twitter.com/LikkleBITCH_/status/1245834140747993090
https://twitter.com/JamesLYork/status/1246153759669006337/photo/1
here is all three
care facility worker escorts yarmulke wearing man to car with medical supplies taken from care facility- nurse intervenes to stop-unsuccessfully
social media
https://twitter.com/LikkleBITCH_/status/1245834140747993090
https://twitter.com/JamesLYork/status/1246153759669006337/photo/1
5:5