>>9048841 LB
>>9049364 LB
>>9042039 PB
>Ask Fauci - How did the polybasic furin cleavage site PRRA get into Covid-19?
>>9046572 PB
Moar articles on the cleavage site and the role of furin.
https://raccoonmedicine.com/wp/2020/03/21/sars-cov-2-ace2-furin/#.XrKvvlDLdYc
Note the date. Comments as interesting as the article.
https://www.virology.ws/2020/02/13/furin-cleavage-site-in-the-sars-cov-2-coronavirus-glycoprotein/
"Furin inhibitors have been studied for their antiviral effects but clinical trial data is limited. So far I have not been able to identify any FDA approved furin inhibitors. Furin is a vital enzyme with hundreds of known substrates, so perhaps this is not surprising. "
https://onlinelibrary.wiley.com/doi/pdf/10.1002/cti2.1073
Furin-mediated protein processing in infectious diseases and cancer
Accepted 13 July 2019
Furthermore, the enzymatic activity of furin is exploited by numerous viral and bacterial pathogens, thereby enhancing their virulence and spread.
In this review, we describe the physiological and pathophysiological substrates of furin and discuss how dysregulation of a simple proteolytic cleavage event may promote infectious diseases and cancer.
>pic related
https://www.ncbi.nlm.nih.gov/pubmed/19057874
Methods Mol Biol. 2008;454:229-36. doi: 10.1007/978-1-59745-181-9_17.
Manipulation of the coronavirus genome using targeted RNA recombination with interspecies chimeric coronaviruses. <---
Targeted RNA recombination has proven to be a powerful tool for the genetic engineering of the coronavirus genome
Key to the two-step method is the efficient selection of recombinant viruses based on host cell switching.
The first step consists of the preparation -using this selection principle- of an interspecies chimeric coronavirus. In this virus the ectodomain of the spike glycoprotein is replaced by that of a coronavirus with a different species tropism.
In the second step this chimeric virus is used as the recipient for recombination with synthetic donor RNA carrying the original spike gene. Recombinant viruses are then isolated on the basis of their regained natural (e.g., murine or feline or bats) cell tropism.
Additional mutations created in the donor RNA can be co-incorporated into the recombinant virus in order to generate mutant viruses.
https://www.edge.org/conversation/william_mcewan-molecular-cut-and-paste
The New Generation of Molecular Tools -- William McEwan [7.21.11]
This afternoon I received in the post a slim FedEx envelope containing four small vials of DNA. The DNA had been synthesized according to my instructions in under three weeks, at a cost of 39 U.S. cents per base pair (the rungs adenine-thymine or guanine-cytosine in the DNA ladder).
The 10 micrograms I ordered are dried, flaky, and barely visible to the naked eye, yet once I have restored them in water and made an RNA copy of this template, they will encode a virus I have designed.